S100A4 A10 in rheumatoid arthritis synovial fibroblasts the expression and significance of.docVIP

 PAGE \* MERGEFORMAT 18 S100A4 A10 in rheumatoid arthritis synovial fibroblasts the expression and significance of Author: Bo dry-ping, Li-Na Zhou, HE Wei-feng, LUO happy, Chen-en Wei, Kuan-Hsing Chen, Hu Xiaohong, Redstone Wen, Fang Fei, Wu Jun [Abstract] Objective: To investigate the normal and rheumatoid arthritis (RA) patients with synovial fibroblast proteome for differential protein analysis of differences in the pathogenesis of RA. Methods: Solid-phase pH gradient (IPG) two-dimensional gel gel electrophoresis (2  DE) separation of normal and RA synovial fibroblasts in patients with total protein; application of two-dimensional electrophoresis gel analysis software (PDQuest 7.3.1) on the 2  DE gel quantitative comparative analysis; application of matrix-assisted laser desorption ionization time of flight mass spectrometry identification of some differences in protein, and used Western Blot, immunocytochemistry validate differentially expressed proteins. Results: Patients with normal and RA synovial fibroblast cell protein 2  DE gel map, respectively, show an average of 896 and 992 points. Expression of 64 protein spots were there is two times more differences. select 9 in RA synovial fibroblasts increases expression of the protein spots identified by mass spectrometry successfully identified calcium-binding protein S100A4 and S100A10 two proteins. by Western Blot, immunocytochemical verification of these two proteins in RA synovial fibroblasts increases expression, consistent with the above, and found that S100A4 in RA synovial fibroblasts in the expression and distribution has changed. Conclusion: The binding protein S100A4 and S100A10 may be involved in RA pathogenesis. S100A4 translocation in the cells may be related to RA synovial fibroblast phenotype changed. [Keywords:] synovial fibroblast; protein group; two-dimensional electrophoresis; rheumatoid arthritis; S100A4 gene; S100A10 gene [Abstract] AIM: To find the differentially expressed