RP-HPLC Determination of alkali content in rat tissues of different Uncaria.docVIP

 PAGE \* MERGEFORMAT 9 RP-HPLC Determination of alkali content in rat tissues of different Uncaria [Abstract] Objective To determine the tissues in rats method Isorhynchophylline. Methods protein precipitation with methanol and then methanol and nitrogen blow drying apparatus after reconstitution Direct determination by RP HPLC. To Waters · RP18 column (4.6 mm × 250 mm, 5 μm) as stationary phase, the front guard column for the Wondasil C18 (4.0 mm × 10 mm, 5 μm), methanol - water (70:30) containing 0.03% triethylamine as mobile phase, flow rate 0.8 mL / min, detection wavelength was 254 nm. carbamazepine to do the internal standard. Results Isorhynchophylline content in rat tissue standard curve equation: Y = 48.558X +0.207 6 (n = 6), r = 0.999 6, 0.16 ~ 16.0 μg / mL a good linear relationship, the average recovery was 99.66%, RSD = 1.56%. Conclusion The method is sensitive, rapid, simple, specific and reproducible. [Keywords:] Isorhynchophylline, rat tissues, determination, HPLC Abstract: Objective To establish a pretreatment mthod of the tissue sample of rat and a determination method of the concentration of isorhynchophylline in tissue of rats by RP-HPLC. Method The tissue sample were treated by adding methanol, and the supernatant was dried by nitrogen gas , the residue was redissolved in methanol, then detected by RP-HPLC directly. Waters · RP18 (4.6 mm × 250 mm, 5 μm) and pre-column Wondasil C18 (4.0 mm × 10 mm, 5 μm) were used as the stationary phase. Methanol-water (70:30) added 0.03% triethylamine was taken as mobile phase with flow rate of 0.8 mL / min. The wavelength of detection was 254 nm. Results The standard curve of Isorhynchophylline concentraton in rat tissues: Y = 48.558 X +0.207 6 (n = 6), r = 0.999 6, it appears a good linear relationship within the range of 0.16 ~ 16.0 μg / mL. The average recovery of was 99.66%, RSD was 1.56%. Conclusion This mehod is sensitive , rapid, simple, specific and reproducible. Keywords:: Isorhynch