RP-HPLC determination Kangguzhengsheng Icariin pills.docVIP

 PAGE \* MERGEFORMAT 6 RP-HPLC determination Kangguzhengsheng Icariin pills [Abstract] Objective: To establish Kangguzhengsheng pills method for determination of icariin. METHODS: RP-HPLC method. Results: In the 0.0618 ~ 0.309μ g icariin showed good linear relationship within the framework of (r = 1.000), the average recovery was 99.55%, RSD = 0.8% (n = 5). Conclusion: The method is simple, reproducible, can be used as method for the determination of the preparation. [Keywords:] RP-HPLC; Kangguzhengsheng pill; Icariin Kangguzhengsheng pills from Rehmannia glutinosa, Epimedium, Cistanche, Woodwardia taste of Chinese herbal medicines such as the composition of compound 9 Shuiwan preparation, there is Bu Yao kidney, strengthening the bones, blood circulation, Lee gas, the function of pain. Of the original quality standards [1] there is no determination methods, in order to more effectively control the underlying quality, and this experiment, try to use RP-HPLC determination of prescription medicines in Epimedium icariin content, method is simple, good reproducibility and control of the drug can be used as quantitative indicators of inherent quality. An instrument and reagent Shimadzu LC-2010A HT high-performance liquid chromatography, UV detector; Shimadzu UV-2450 UV spectrophotometer; sartorius electronic balance; KQ-100 ultrasonic cleaner; methanol chromatography pure reagent, other reagents of analytical pure; icariin reference substance (batch 110737-200312 for the determination of use, purchased from the Chinese pharmaceutical and biological products); Kangguzhengsheng pill (commercially available). 2 Results and discussion 2.1 Preparation of reference substance solutions that take precise amount of icariin reference substance, add methanol produced per 1ml solution containing 30.9μ g. 2.2 Preparation of solution for the test items to take this product 50 pills, and research fine, take 2g, precise, said determined set 100ml bottle with cone-shap