Saikosaponin d in human hepatoma HepG2 cell proliferation and cell cycle.docVIP

 PAGE \* MERGEFORMAT 11 Saikosaponin d in human hepatoma HepG2 cell proliferation and cell cycle Of: Zhang Guoping, INTEGRATED Wang Lan days, Tongchuan Ming, Jing Min [Abstract] Objective saikosaponin d (SSd on human hepatoma HepG2 cell proliferation and cell cycle. Methods (1) human hepatoma HepG2 cells in normal culture, (2), thiazolyl blue (MTT test treatment effects of different concentrations of SSd HepG2 cells after 72 h and 10mg / L in HepG2 cells at different time SSd effect on HepG2 cell growth index, (3) flow cytometry (FCM detection of HepG2 cells SSd handle the distribution of the cell cycle after 48h. The results could inhibit SSd HepG2 cells to 10mg / L of SSd most obvious effect of the inhibitory effect of 48h. FCM analysis showed that the experimental group after 48h SSd role in S phase cells were markedly reduced (P lt;0.01. Conclusion of human hepatocellular carcinoma SSd the proliferation of HepG2 cells inhibited cell cycle and allows the number of cells in S phase decreased. [Keywords:] saikosaponin d; HepG2; proliferation, cell cycle Abstract: Objective To study the effects of saikosaponins d (SSd) on proliferation and cell cycle in the human hepatocellular carcinoma cell line, HepG2 cell line. Methods HepG2 cells were cultured and treated with SSd. The cell growth rate was measured by monotetrazdium (MTT assay and the cell cycle was determined by the flow cytometry (FCM) analysis. Results The SSd obviously inhibited the proliferation of HepG2 cell line in a concentration and time dependent manner, the maximum response of the cells to SSd was at the concentration of 10mg / L and the treatment time point of 48h. Compared with the control, the cells treated with 10mg / L SSd for 48h significantly reduce cell numbers in S phase (P lt;0.01). Conclusion The SSd could inhibit the proliferation of HepG2 cell line and arrest its cell cycle in S phase. Keywords:: saikosaponins d; HepG2; proliferation; cell cycle Liver cancer is