Sal B in vitro differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells in experimental study.docVIP

 PAGE \* MERGEFORMAT 18 Sal B in vitro differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells in experimental study Author: Xu Xiumei, Guomao Juan Zhao FAN Ying-chang [Abstract] Objective To observe the Chadan acid B (SalB) interfere with in vitro differentiation of bone marrow mesenchymal stem cells (MSCs) to differentiate into cardiomyocyte-like cells and its mechanism. Methods Isolation and identification of rat MSCs, inducing them grouped: SalB group (final concentration of 250 μ g / L), 5 - azacytidine group (final concentration of 10 μ mol / L), SalB combined 5-aza group ( final concentrations were 250 μ g / L and 10 μ mol / L), induced 24 h, continued to train for 4 weeks to observe the morphological changes were identified by immunocytochemistry cardiac-specific troponin T (cTnT) expression, real-time fluorescence Quantitative RT-PCR to detect early myocardial gene NKX2.5, GATA-4, Desmin, and a-actin mRNA expression. The results of MSCs induced volume increase, proliferation slowed down, and the emergence of myotube-like structure; immunohistochemistry results showed that MSCs after induction of the expression of cardiac-specific protein, cTnT, as compared with 5-aza group, SalB group of cTnT expression in the lower , SalB combined 5-aza group of cTnT expression was elevated; real-time quantitative RT-PCR results showed that all groups were early gene expression of cardiac differentiation, SalB combined 5-aza group induced early differentiation of MSCs in myocardial gene expression was significantly higher than the other two group. Conclusion SalB can be induced in vitro directed differentiation of MSCs into cardiomyocytes-like cells, combined with 5-aza-induced MSCs can significantly enhance the ability of cells to the myocardium. [Keywords:] Sal B; bone marrow mesenchymal stem cells; vitro; immunocytochemistry; real-time quantitative RT-PCR Bone marrow-derived mesenchymal stem cells (MSCs), Department of autologous sou