Scarce in spontaneously hypertensive rats and apoptosis of retinal microvascular.docVIP

 PAGE \* MERGEFORMAT 24 Scarce in spontaneously hypertensive rats and apoptosis of retinal microvascular Of: Xiao-Yan Huang, Chen Bi-new, Gao Zhan, Zhang Mingying, Wang Sai Bin, Yang Industry [Abstract] Objective?: Study in spontaneously hypertensive rats (SHR) the occurrence of apoptosis of retinal capillary cells, to explore the apoptosis of retinal microvascular disease and hypertension in the relationship between the retinopathy study the pathogenesis of hypertension morphological basis. Methods: 10 male spontaneously hypertensive rats (SHR group) and 10 male normotensive (WKY) rats (WKY group) at 13 weeks and 18 weeks of age to take the eye retina digestion stretched to do using terminal deoxynucleotidyl transferase-mediated dUTP nick translation method (TUNEL) assay of apoptosis of retinal microvascular changes, HE staining and image analysis, dynamic morphological changes of retinal capillaries and retinal capillary area and density determination of the change . Results: TUNEL method labeling showed SHR rat retinal microvascular the week there were positive cells and positive cells 18 weeks old rats compared with 13 weeks old rats was significantly increased; WKY rats, no TUNEL-positive cells in retinal microvascular . vessels stretched SHR group of HE staining showed that some capillary occlusion, loss of endothelial cells and pericytes appeared acellular capillaries, capillary density was significantly lower than WKY. Conclusion: In the course of development of hypertension, retinal capillary endothelial cells and pericytes apoptosis can occur, accompanied by sparse microvascular apoptosis in hypertensive retinopathy may play an important role in the process. [Keywords:] hypertension retinopathy; apoptosis; microvascular scarce Abstract:? Objective: To observe the apoptosis of retinal capillary cells in spontaneously hypertensive rats and study the relationship between apoptosis and hypertensive retinopathy, in order to provide morphologi