肝门部胆管癌细胞系高转移亚系的建立.pdfVIP

? 688 . ·实验研 肝门部胆管癌细胞系高转移亚系的建立 用普生吴小鹏 [摘费 1 目的 利用肝门都胆管癌服发病灶建立的细胞系接种裸鼠牌脏，筛选出高转移亚系。 方法 将 FRH-0201 制成细胞悬液，接种 7 只 BALB/C 裸鼠脾脏。将转移的肿瘤行组织块培养，筛 选出细胞亚系。 FRH孔再次接种裸鼠，将转移瘤再次培养，筛选出细胞亚系 FRH-B 。观察细胞形 态学、核型变化、肿瘤标志物、角蛋白染色及细胞生长曲线。结果 FRH-A 及 FRH-B 细胞系为梭 形和圆形细胞，生长旺盛。电镜显示典型恶性细胞特征。细胞倍增时间为 2 1. 8 h ，染色体众数 19 条，主流施围 18-44 条。细胞上清液 CA125 显著增高。细胞角蛋白染色阳性。 FRH-A 细胞再次 接种裸小鼠，转移发生率 100% ，肿瘤生长迅速，转移发生快。结论 FRH-A 及 FRH-B 细胞系来源 于 FRH-0201 的高转移的亚系。 {关键词} 肝门部胞管癌; 转移 Establishment and studies on characreristiωof high metastatuc su协 cellline of hilar cholangiocarcinoma ZHOU ]in-shen嚣 ， WU Xiao- 件ng. Depaγtment 01 General Surgery, Qilu Hospi，ω l olShando饵g Ulli? versity,]i nan 250012, China [Abstractl Objective To establish a high metastatic sub-cell line and observe their biological characteristics by inocu1ating cholangiocarcinoma cellline (FRH-0201)on spl悦n of nude mice. Methods Each of 7 mice wωinjected O. 1m! of FRH-0201cell suspension (1. 5 x 107 cell) into the pole of the spleen. Metastatic tumor was cultured again. The sub-ce111ine cell(FRH-A) was injected into spleen mice and metastatíc tumor formation rate was observed. Carbohydrate antigens and karyotype were examined. Growth curves of all celllines were figured. Results FRH唰 A cel1 line growth bloom. morphologic施1 of the metastatic tumor cel1 were only rotundity and shuttle. pop u1ation doubling tíme was 21.8 h, chromosome ∞unts showed model number was 19 , distribute frome 18 to 44. Tumor marker苗( CA125) were rise tω. 丁ransmission electron mícrograph of the cel1, showíng large malformed nucleolus, desmosome junction and microvil1í on theír surfaces. When the FRH-A cells were inoc吐ated ín mi