梅毒螺.docVIP

梅毒螺旋体抗体检测方法分析 摘要 目的 人体感染梅毒螺旋体后，具有高度传染性，血清梅毒螺旋体抗体检测对梅毒诊断和疗效进行分析很重要，比较梅毒酶联免疫吸附试验（TP-ELISA）、梅毒甲苯胺红不加热血清试验（TRUST）、梅毒螺旋体明胶颗粒凝集试验（TPPA）三种检测方法，探讨如何合理利用这3种方法来检测梅毒抗体。 方法 对龙岗中心医院350例梅毒患者、100例非梅毒患者、50例正常人的血清标本进行TP-ELISA、TRUST、TPPA三种方法联合检测并进行分析。 结果 153例一期梅毒患者阳性检出率分别为97.4%、83.7%、98.9%；185例二期梅毒患者阳性检出率均为97.8%、96.2%、100%；22例潜伏期梅毒患者阳性检出率分别为95.5%、72.7%、100.0%；100例非梅毒患者阳性率分别为1%、4%、1%；50例健康体检者的阳性率均为0。 结论 三种方法都是梅毒检测的快速方法， TP-ELISA适合批量筛查试验而且对早期梅毒灵敏度也较高，适合早期特异性诊断，TRUST 可以测定抗体滴度，疗效观察优于TP-ELISA和TPPA，TPPA的灵敏度特异性最高，适合作为确证试验。 关键词 梅毒螺旋体 检测方法 检出率 试验 Syphilis helicoid antibody test method analysis Abstract objective Human infections after syphilis helicoids,Highly infectious, Serum syphilis helicoid antibody test for syphilis diagnosis and curative effect analysis is very important, HYPERLINK app:ds:compare \t compare syphilis ELISA test (TP-ELISA),Syphilis armour aniline red do not heat serum test (TRUST)with Syphilis helicoid gelatin particles agglutinate experiment (TPPA) these three kinds of methods, Discusses how to reasonable use of the three methods to detect syphilis antibody. Methods 153 cases of syphilis patients a positive detection rate of 97.4%, 83.7% and 98.9% respectively; 185 cases of patients with two positive detection rate of syphilis are 97.8%, 96.2%, 100%; 22 cases of syphilis patients positive incubation period detection rate of 95.5%, 72.7% and 100.0% respectively; 100 cases of syphilis positive patients were 1%, 4% and 1%; 50 cases of all the healthy check-up of positive for 0. Conclusions Three methods are syphilis detection method of fast, TP-ELISA suitable for mass screening test for early syphilis and higher sensitivity, Suitable for specific diagnosis early, TRUST can be determined antibody concentration degree, Clinical observation is better than the TP-ELISA and TPPA, The sensitivity of the highest TPPA specificity, As a test for confirmation. Keywords Treponema pallidum test method relevance ratio test 比较梅毒酶联免疫吸附试验（TP-ELISA）、梅毒甲苯胺红不加热血清试验（TRU