Simultaneous determination of 3 kinds of pear seeds endogenous hormones.docVIP

 PAGE \* MERGEFORMAT 15 Simultaneous determination of 3 kinds of pear seeds endogenous hormones Study: Teacher Jie Guo Yan Liang Liya Wang Jidong Zhang Lina Li Wu Caie [Abstract] established Simultaneous determination of 3 kinds of pear seeds endogenous hormone gibberellin (GA3), auxin (IAA) and abscisic acid (ABA) content of the method. Using C18 reversed phase column, column temperature of 35 ℃, methanol water (containing 1% acetic acid) (4:6, V / V) as mobile phase, flow rate 1 mL / min, detector wavelength began to 254 nm, 2.5 min when the switch to the 210 nm wavelength to 3.8 min, GA3 after the end of the peak, once again switch back to 254 nm wavelength determination of IAA and ABA.GA3, IAA and ABA, the average recoveries were 92.8%, 91.6% and 94.7%. for the rapid and accurate separation and determination Pear seeds hormone provides a reliable method. [Key Words] high performance liquid chromatography; endogenous hormone; pear; seed Abstract A high performance liquid chromatographic (HPLC) method for the simultaneous determination of three kinds of endogenous hormone gibberellin (GA3), auxin (IAA) and abscisic acid (ABA) content in seeds of post harvest Yali pear was described. The chromatographic separation was carried out on a reverse phase C18 column using methanol: water (containing 1% acetic acid) = 4:6 as the mobile phase at a column temperature of 35 ℃. The flow rate was 1 mL / min. UV detection at 210 nm was used for separation GA3 from 2.5 min to 3.8 min, while detection at 254 nm was used for the remaining separations. Average recoveries were 92.8%, 91.6% and 94.7% for GA3, IAA and ABA. It shows a reliable method for rapid, accurate separation and determination endogenous hormone in Yali pear seeds. Keywords High performance liquid chromatography; Endogenous hormones; Yali pear; Seed 1 Introduction The fruit maturation is a complex physiological and biochemical processes, plant hormones and fruit development and maturity, w