Sodium nitroprusside-induced apoptosis of K562 cells and telomerase activation during STAT3-P38MAPK expression of hTERT-mRNA.docVIP

 PAGE \* MERGEFORMAT 25 Sodium nitroprusside-induced apoptosis of K562 cells and telomerase activation during STAT3/P38MAPK expression of hTERT-mRNA Author: Zhou columns, Ya-Ping Lü, Lu Huo-Xiang, Lian-Nü Qiu, Wen-Song Wang, Lin Huijun, Liu Jiandong 【Abstract】 In order to study exogenous nitric oxide donor sodium nitroprusside (SNP)-induced apoptosis of K562 cells and the activation of STAT3 and P38MAPK telomerase hTERT-mRNA expression in the changes of sodium nitroprusside-induced apoptosis of K562 cells, mechanism is Annexin-V/PI double labeling, DNA fragments in situ end-labeling method, DNA gel electrophoresis, DNA content and cell cycle analysis to detect apoptosis. Measured by flow cytometry after the intervention of K562 cells by sodium nitroprusside phosphorylation P38MAPK and phosphorylated STAT3 expression, while taking advantage of real-time fluorescence quantitative PCR detection of telomerase hTERT-mRNA expression changes. The results showed that: K562 cells were treated with sodium nitroprusside after the role of the typical morphological changes, DNA fragmentation, indicating that sub-G1 peak Ⅱ and increased significantly. Annexin-V/PI and in situ end-labeling DNA fragments increased expression. These are confirmed NO can induce apoptosis of K562 cells, most cells were arrested in the G0/G1 phase. SNP-induced apoptosis of K562 cells, the phosphorylation P38MAPK and phosphorylated STAT3 expression with increasing concentration of SNP showed the first increase and then decreased; K562 cells and 2.0 mmol / L SNP in different incubation time, the expression of phosphorylated P38MAPK reached its peak in 12 hours and continued to 48 hours, 72 hours after the down-expression; phosphorylation of STAT3 expression in 24 hours and up to the peak expression after 48 hours that is significantly decreased; telomerase hTERT-mRNA expression in the role with the SNP concentration increased and prolonged significantly reduced. Conclusion: SNP can ind