Steroid hormone GC MS analysis of keto derivatives of Research Progress_0.docVIP

 PAGE \* MERGEFORMAT 31 Steroid hormone GC MS analysis of keto derivatives of Research Progress Of: Fang Kai Pan Xuejun Wang Yu Liang Liu Jing Huang Bin Gao Jian Pei [Abstract] steroid hormone with its unique physiological and biochemical effects have attracted much attention, gas chromatography mass spectrometry has become the steroid hormone analysis testing an important tool. Since steroid hormones containing hydroxyl, ketone and other polar functional groups, GC detection sensitivity of MS analysis is not high, chromatographic separation peak shape is poor, to be derived through technology to convert it into less polar, volatile, high thermal stability of the compounds to improve the chromatographic peak shape and improve the detection sensitivity so applicable in the GC MS trace analysis. At present, steroid hormones hydroxyl containing active hydrogen atoms of functional group derivatization technology is more mature, but the keto derivative is still a lack of systematic research. In this paper, steroid hormone-keto derivative Technology were reviewed, mainly introduced enolate silylation, oximation silylation derivatization techniques such as keto reaction principle, application, and the current problems and future research directions are discussed and prospects for the steroid hormone-keto derivative technology to provide reference. [Keywords:] steroid hormone; ketone; derivatization; gas chromatography mass spectrometry 1 Introduction Steroid hormones is cholesterol synthesis of biologically active, soluble, low molecular weight and have a steroidal hormone function [1,2]. Steroid hormone with its unique physiological and biochemical role of a legitimate field of applied human and animal medicine , is the treatment or detection of human and animal diseases, an important basis for some [3]. but because of steroid hormones can greatly promote animal growth, since the 20th century, since the 80 in livestock [4,5]. Meanwhile, some steroids h