Taishan 4 leaves of medicinal plants DNA extraction method of parameters.docVIP

 PAGE \* MERGEFORMAT 12 Taishan 4 leaves of medicinal plants DNA extraction method of parameters Author: Ren-Jiu, Wang Guilong, Gong Yuan Wei, Hao Gang-Ping, Wang Cai [Abstract] Objective To study the DNA extraction methods on the four leaf parameters Taishan DNA quality. Methods of DNA extraction carried out by UV spectral analysis, the total DNA electrophoresis and RAPD analysis. The results of different methods of DNA extraction quality and yield significant differences. Conclusion The results show that modified CTAB method and modified SDS method better. [Keywords:] Tarzan four leaf ginseng genomic DNA DNA extraction method Taishan four leaf parameters Codonopsis lanceolata Benth.et Hook., Belong to Campanulaceae Codonopsis, Taishan, one of the four-name drugs in order to root for the drug, with a blood-pass milk, Yin lungs, Yiwei Sheng Jin’s effect [1]. This study was designed using RAPD molecular marker technology to build four leaf parameters of different origin DNA fingerprints for the four-leaf ginseng provide molecular basis for the authenticity of identification. High-quality genomic DNA is a reliable marker to obtain the results of the premise. Taishan 4 leaf ginseng polysaccharide, protein content was high, difficult to remove, thus affecting the purity and concentration of DNA. This study improved the traditional SDS method and CTAB method. Using improved methods of DNA extracted from the study of molecular biology can be used as the template, which for the RAPD molecular markers and follow-up study of molecular biology has laid an important foundation. 1 Equipment MyCycler gradient PCR instrument (Bio-rad Company), centrifuge (Sigma 3K30), DNA Centrifugal Vacuum Dryer (Savan t). Taishan 4 leaf ginseng leaves were collected from Taishan medicinal botanical gardens, stored in -70 ℃ refrigerator spare. CTAB (Shanghai Biological Engineering Technology Co., Ltd.), SDS (Shanghai Biological Engineering Technology Co., Ltd.), PVP (Shanghai Biol