The establishment of inbred rat model of acute rejection after liver transplantation experience.docVIP

 PAGE \* MERGEFORMAT 21 The establishment of inbred rat model of acute rejection after liver transplantation experience [Keywords:] Rats, inbred strains; liver transplantation; disease models, animal; graft rejection Establishment of acute rejection model of orthotopic liver transplantation in inbred rats [Abstract] AIM: To establish the orthotopic liver transplantation models in inbred rats and judge the grades of acute rejection. METHODS: Surgical skills were trained by using closed colony SD and Wistar rats, followed by DA to LEW orthotopic liver transplantation with Kamadas twocuff technique . The grades of acute rejection were judged by their clinical manifestations and Banff schema. RESULTS: Fifteen models of DA to LEW liver transplantation were established, the success rate being 86.7%. The causes of death were hemorrhage and thrombosis of suprahepatic and infrahepatic vena cava and obstruction of biliary tract. Ⅰ grade rejection occurred at 3rd day and reached the peak at 7th day after transplantation. Remaining models died of Ⅲ grade rejection besides of postoperative complication within 12 d after transplantation. CONCLUSION: The acute rejection of DA to LEW orthotopic liver transplantation model is stable and intensive. It can be effectively used to study allograft rejection and tolerance. But it is difficult to establish the kind of orthotopic liver transplantation models in inbred rats because of their own tissue characteristics. [Keywords] rats, inbred strains; liver transplantation; disease models, animal; graft rejection [Abstract] Objective: To investigate the inbred rat orthotopic liver transplantation model and determine the intensity of rejection. Methods: Kamada two-cuff technique, the use of closed colony SD and Wistar rats modeling skills training, in the this basis, the establishment of inbred DA-LEW rat liver transplantation model, based on clinical manifestations and Banff criteria to judge the intensity of rejection. Results: A