Variant of E. coli detection methods in molecular biology research.docVIP

 PAGE \* MERGEFORMAT 31 Variant of E. coli detection methods in molecular biology research Keywords: Escherichia coli O157; molecular biology; Bacterial typing techniques E. coli is one of the most common micro-organisms, widely distributed in nature, also exist in animals. It has easy to cultivate, grow fast, easy variation and so on. EHEC (enterohemorrhagic E. coli, EHEC) is a variant of E. coli, an outbreak of epidemic in many countries. At present the United States, Canada and other countries, the emergence of new drug-resistant variants [1], has led to many deaths. Therefore, pathogenic variants of E. coli caused by the close attention of scholars home and abroad, and variants of E. coli conducted in-depth study. Writer EHEC serotype O157: H7, for example, its molecular biological detection methods are reviewed, in order to diseases caused by bacteria of the prevention, diagnosis and treatment reference. 1 EHEC Basic Overview E. coli O157: H7 on several occasions around the world, especially the developed countries cause widespread outbreak. Infection mainly cause diarrhea, hemorrhagic colitis (hemorrhagic colitis, HC), and often associated with hemolytic uremic syndrome (hemolytic uremic syndrome, HUS), thrombotic thrombocytopenic purpura formation (thrombotic thrombocytopenic purpura, TTP) complications, serious threat to human life and health [2]. Of human infection with the bacteria mainly through consumption or contact with contaminated beef, vegetables, fruit and water and so on. EHEC strains can produce toxins, the genes encoding these toxins, including Shiga-STZ-1 gene (st x1), Shiga Abamectin 2 gene (st x2), E. coli adhesion and to eliminate genes (eae), and enteric-coated blood hormone gene ( ehx) and so on. E. coli O157: H7 associated with human disease, the most common serotypes of bacteria. In 1982, the American scholar, the first time from patients with hemorrhagic diarrhea, stool samples isolated from ba