Xian soup on mice Lewis lung cancer effect of.docVIP

 PAGE \* MERGEFORMAT 19 Xian soup on mice Lewis lung cancer effect of Author: Cao Yang Pang Jie Hu Yan Li, Yan Chen Lin Xiang Yao-Ni Chen both Kaiji Chen Rui Chen Xue-xin Tan Deep [Abstract] [Objective] To have a spleen Qingfei, resolving phlegm and blood stasis and the role of Chinese herbal compound sen soup on growth inhibition of murine Lewis lung cancer and its related molecular mechanism. [Methods] 50 Lewis lung tumor-bearing mice were divided into cents soup high, medium and low-dose group (dose, respectively 1.747,0.874,0.437 g * kg-1 * d-1), cyclophosphamide (CTX) Group (dose of 20mg * kg-1 * d-1), tumor-bearing model group; calculate the average tumor mass in mice in each group and the inhibitory rate; Preparation of single cell suspension using flow cytometry mice in each group Lewis lung cancer cell cycle and apoptosis rate; using immunohistochemical staining of each group of mice Lewis lung cancer, bcl  2 gene expression. [Results] cents soup high, medium and low-dose group and CTX group were lower than model group, the tumor mass, tumor cell apoptosis rate were higher than model group (P amp;lt;0.05 or P amp;lt;0.01), cents fish soup in each group as the dose increases, the inhibition rate and apoptosis rate of tumor cells gradually increased. Cents fish soup high, medium and low-dose group and CTX group in the proportion of cells in S phase was lower than the model group (P amp;lt;0.05 or P amp;lt;0.01), showing clearly the phenomenon of G0/G1 phase arrest. Sin soup each dose group bcl  2 staining intensity index was significantly lower than model group (P amp;lt;0.01). [Conclusion] Sin soup could inhibit the growth of mouse Lewis lung cancer, the role of the mechanism may be by reducing expression of bcl  2 so as to promote tumor cell apoptosis. [Keywords:] cents soup / pharmacology lung neoplasms / TCM therapy apoptosis regulation of gene expression Cell Culture Lung cancer is a serious harm to human health of the malignant tumors