口服当归补血汤后阿魏酸在大鼠体内的组织分布.docVIP

　　口服当归补血汤后阿魏酸在大鼠体内的组织分布 作者：王永禄, 王丽瑶，陈国广，张东旭，韦萍 【摘要】 　　目的研究大鼠灌胃给予当归补血汤后阿魏酸在体内的组织分布特性。方法采用高效液相色谱法；色谱柱为Kromasil C18（4.6 mm×150 mm,5 μm）；流动相为0.2%甲醇乙腈-0.2%磷酸0.2%三乙胺水溶液（18：82）；检测波长322 nm；柱温40℃；流速0.8 ml/min。结果各组织中的阿魏酸在10～400 ng范围内与峰面积呈良好的线性关系；回收率平均为87.97%±1.95%；日内和日间的RSD分别1.92% ～3.87% 和3.21%～6.39% 之间。阿魏酸在各组织器官中浓度差异较大。结论大鼠灌胃给予当归补血汤后阿魏酸主要在肝脏和肾脏分布，阿魏酸不能通过血脑屏障。 【关键词】 当归补血汤； 阿魏酸； 反相-高效液相色谱； 组织分布； 大鼠 　　Abstract:ObjectiveTo investigate the tissue distribution of ferulic acid in rats after oral administration of TangkuEi blood-supplementing decoction.MethodsHPLC n: Kromasil C18（150mm×4.6mm ，5μm）; mobile phase: 0.2%methanol acetonitrile-0.2% phosphoric acid 0.2%triethylamine solution（18：82）; detection ; temperature l/min.ResultsThe linear ranges of ferulic acid in the heart, liver, spleen, lung and kidney ajor distribution organs of ferulic acid in rats are liver and kidney. Ferulic acid can not pass though the blood-barrier. 　　Key enting decoction ; Ferulic acid; Tissue distribution； Rats 　　Tangkuei Blood-Supplementing Decoction has been ia, female irregular menstruation and amenorrhoea in the traditional Chinese medicine for hundreds of years. Previous studies have demonstrated that Tangkuei Blood-Supplementing Decoction can inhibit lipid peroxidation, maintain activities of SOD and GSH, enhance cell ability against oxidation, improve immune function, and lessen ischemia/reperfusion injury in the heart and the brain［1］. Ferulic acid, one of the important ponents of Tangkuei Blood-Supplementing Decoction［2］, diminished the production of H2O2 in hepatocytes and protected membrane lipid after ischemia/reperfusion. It also plays a role of dilating blood vessel［3］, inhibiting platelet aggregation, inhibiting inflammatory reaction, anti-oxydation and cleaning free radical, acting on eliminating and inhibiting. In the present study, the tissue distribution of ferulic acid in rats ance liquid chromatography after oral administration of Tangkuei blood-supplementing decoction.