异氟醚后处理对盲肠结扎穿孔大鼠急性肺损伤的保护作用.docVIP

　　异氟醚后处理对盲肠结扎穿孔大鼠急性肺损伤的保护作用 作者：徐文莉1，何俊1，曾因明1，庞庆丰 【摘要】 目的 观察异氟醚(isoflurane, ISO)后处理对盲肠结扎穿孔术(cecal ligation and puncture, CLP)后大鼠急性肺损伤(acute lung injury,ALI)的保护作用。方法 32只SD雄性大鼠随机分为4组(每组n=8)：正常组(N组)，未行任何处理;假手术组(Sham组)，单纯行开腹关腹术;盲肠结扎穿孔术组(CLP组)，行CLP建立大鼠ALI模型;异氟醚组(ISO组)，建立CLP模型后4 h腹腔注射液态异氟醚2 ml/kg。术后12 h各组动物腹主动脉放血处死，右肺进行病理切片和肺湿/干比(DA)和髓过氧化物酶(MPO)的含量;左肺行支气管肺泡灌洗，取上清液测总蛋白、肿瘤坏死因子α(TNF-α)和白细胞介素10(IL-10)的含量。结果肺组织病 理学 检测结果显示CLP组大鼠肺组织损伤严重，ISO组肺组织损伤较轻。与N组比较，CLP组和ISO组中SOD含量降低(Plt;0.01)，其余检测指标均增加(Plt;0.01)。与CLP组比较，ISO组SOD和IL-10含量较高(Plt;0.01);其余检测指标均低于CLP组(Plt;0.05或Plt;0.01)。结论 在本实验条件下，大鼠腹腔注射液态ISO可发挥抗炎、抗氧化作用，对CLP所致大鼠ALI有部分保护作用。 【关键词】 急性肺损伤;肺保护;异氟醚;后处理;盲肠结扎穿孔 　　Abstract: Objective To investigate the protective effects of isoflurane (ISO) post-treatment on acute lung injury (ALI) induced by cecal ligation and puncture (CLP) in rats. Methods 32 male Sprague-Daly divided into 4 groups (n=8 each): normal (N) group, ent; Sham-operated control (Sham) group, ere incision and closure of the abdomen; cecal ligation and puncture (CLP) group, established as rat model of induced ALI; and isoflurane (ISO) group, l/kg ISO 4 h after CLP. The animals inal aorta 12 h after CLP. The right lungs oved for pathological dissection; determination of lung ination of the content of superoxide dismutase (SOD), malondialdehyde (MDA) and myeloperoxidase (MPO) in the pulmonary homogenate. Follo the bronchoalveolar lavage fluid (BALF) ined for the total protEIn level, the content of tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10). Results The pathological results shoeters increased (Plt;0.01), as pared to the N group. The SOD and IL-10 contents ent, intraperitoneal injection of ISO had anti-inflammatory and anti-oxidative effects, CLP-induced ALI in rats. 　　Key ent; cecal ligation and puncture 　　急性肺损伤(acute lung injury, ALI)是急性呼吸窘迫综合征(acute respiratory distress syndrome, ARDS)的早期阶段，是临床常见的危重病症。它是损伤因素直接或间接损伤肺毛细血管内皮细胞，导致内皮细胞功能障碍，肺毛细血管通透性增高的血管渗漏综合征[1-2]。其致病因素有多种，其中脓毒症排在首位[3]。目前 治疗 ALI的方法有病因治疗、体液管理、通