当归补血汤对荷瘤小鼠的影响及对环磷酰胺化疗的增效减毒作用.docVIP

　　当归补血汤对荷瘤小鼠的影响及对环磷酰胺化疗的增效减毒作用 作者：袁国红, 庞晓静 马鹤超 【摘要】 研究当归补血汤对肿瘤的抑制作用及其与免疫功能的关系，并探讨其对环磷酰胺（cytoxan, CTX）化疗的增效减毒作用。 方法：以荷EL-4瘤株C57BL/6纯系小鼠作为病理模型，皮下肿瘤接种（1×106个肿瘤细胞），7 d时随机分为4组，肿瘤模型组，当归补血汤（Danggui Buxue Tang, DBT）组［24 g/（kg·d）］，CTX组［7.5 mg/（kg·d）］，DBT+CTX组，另设正常对照组。每天游标卡尺测定肿瘤长短径，并观察荷瘤小鼠生存时间，以检测DBT的抑瘤作用；免疫学方法（迟发型超敏反应、空斑形成细胞、细胞毒T细胞及 自然 杀伤细胞的杀伤活性，TNF-α活性及外周血、骨髓有核细胞计数等）检测DBT的抑瘤作用与机体免疫功能的关系及其对CTX化疗的增效减毒作用。 结果：接受相应处理15 d后，与肿瘤模型组比较，DBT、CTX和DBT+CTX均可显著减缓小鼠肿瘤生长速度（Plt;0.05），明显延长荷瘤小鼠生存时间（Plt;0.05）。与肿瘤模型组比较，DBT组和DBT+CTX组各项免疫学指标增强（Plt;0.05），CTX组各项免疫学指标明显降低（Plt;0.05）。与CTX组比较，DBT+CTX组各项免疫学指标均有大幅度改善（Plt;0.05）。结论：DBT可减缓肿瘤生长速度，延长荷瘤小鼠生存时间，其抑瘤作用可能与其激活机体免疫功能作用有关，并对CTX化疗有增效减毒作用。 【关键词】 抗肿瘤药; 环磷酰胺; 免疫学; 小鼠 Objective: To study the inhibitory effect of Danggui Buxue Tang (DBT), a pound traditional Chinese herbal medicine for supplementing blood, on tumor groor-bearing mice after inoculation of EL-4 cells, and its immune mechanism as ent or-bearing mice after inoculation of EL-4 cells. The mice ly divided into four groups after 7 days of the inoculation: untreated group, DBT-treated group ［24 g/(kg·d)］, CTX-treated group ［7.5 mg/(kg·d)］ and DBT plus CTX-treated group, al mice as control. Inhibitory rate of tumor groe, immune function and variability of blood cells easured in the mice during the experiment. Results: After treatment of relevant interventions for 15 days, the tumor in the DBT-treated group, CTX-treated group and DBT plus CTX-treated group gree in the DBT-treated group, CTX-treated group and DBT plus CTX-treated group mune indexes in the DBT-treated group and DBT plus CTX-treated group proved (Plt;0.05), mune indexes in the CTX-treated group mune indexes in the DBT plus CTX-treated group proved (Plt;0.05). Conclusions: DBT can enhance the immune function in tumor-bearing mice and the inhibitory effect of DBT on tumor gromune response. DBT can also increase the therapeutic effects and reduce the side effects of CTX. Keyide; immunology; mice 肿瘤患者常伴有免疫功能低下，且临床又常以放、化疗作为其常规 治疗