气管插管快速灌注博莱霉素复制大鼠肺间质纤维化模型.docVIP

　　气管插管快速灌注博莱霉素复制大鼠肺间质纤维化模型 作者：张伟, 鲁香凤, 张晓梅, 吴建军, 姜良铎 【摘要】 研究大鼠肺间质纤维化的造模方法。 方法：模型组大鼠气管插管灌注不同剂量（7、6、5、3.4、2、1 mg/kg体质量）的博莱霉素A5-生理盐水，对照组给予等体积生理盐水，观察大鼠生存情况、肺组织病理，并深入研究博莱霉素1 mg/kg体质量组，观察大鼠体质量变化，肺组织病理，检测第14天、28天、45天时大鼠肺系数以及血清中转化生长因子β1（transforming grog/kg体质量气管内灌注博莱霉素A5-生理盐水可以造成大鼠肺组织纤维化改变。深入研究发现，与假手术组比较，1 mg/kg体质量组大鼠肺组织病理14 d已出现明显的纤维化改变，28 d已形成弥漫性纤维化，45 d时纤维化程度进一步加重。与假手术组比较，模型组3、7、14 d体质量降低（Plt;0.01），21 d体质量虽有所下降，但差异无统计学意义（P＞0.05）；14、28、45 d时肺系数升高（Plt;0.01），血清TGF-β1水平从28 d时开始升高（Plt;0.05，Plt;0.01），血清PDGF水平45 d时升高（Plt;0.05）。1 mg/kg体质量组造模死亡率较低。结论：博莱霉素1 mg/kg体质量气管插管快速灌注法可以成功复制大鼠肺间质纤维化模型。 【关键词】 肺纤维化; 模型, 动物; 博莱霉素; 大鼠 Method: Different doses (7, 6, 5, 3.4, 2, 1 mg/kg) of bleomycin A5-saline ycin-treated group through tracheal intubation, and rats in sham-operated group e volume of saline. The living state and lung pathology of the rats g/kg bleomycin-treated group, and the changes of body ing gro easured on the 14th, 28th and 45th day of the experiment. Results: The study demonstrated that infusing large doses of bleomycin A5 quickly through tracheal intubation had a high mortality, and infusing 1 mg/kg quickly could successfully develop an animal model of pulmonary fibrosis. pared -operated group, fibrosis g/kg bleomycin A5-treated group after 14 days of experiment, diffuse fibrosis ent, and the fibrosis became more severe after 45 days of experiment. The body ycin-treated group ent as pared -operated group (Plt;0.01). Tent, the body ycin-treated group and the sham-operated group (Pgt;0.05). Lung quotient ent (Plt;0.01), the level of serum TGF-β1 began to increase since 28 days after the experiment (Plt;0.05, Plt;0.01), and the level of serum PDGF also increased gradually 45 days after the experiment (Plt;0.05). And the mortality rate of 1 mg/kg bleomycin A5-treated group ycin A5-treated groups. Conclusion: A rat model of pulmonary fibrosis can be duplicated successfully by infusing 1 mg/kg bleomycin A5 quickly through tracheal intubation. Keyonary fibrosis; model,