Degradation Behaviors of Thai Bombyx mori Silk Fibroins Exposure to Protease Enzymes.docVIP

Engineering, 2013, 5, 61-66 /10.4236/eng.2013.51010 Published Online January 2013 (/journal/eng) Degradation Behaviors of Thai Bombyx mori Silk Fibroins Exposure to Protease Enzymes Chuleerat Wongnarat, Prasong Srihanam * Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham, Thailand Email: prasong.s@msu.ac.th * Received November 11, 2012; revised December 11, 2012; accepted December 20, 2012 ABSTRACT The degradation behaviors of Thai Bombyx mori called Samrong and Nanglai silk fibroins exposure to protease en- zymes; protease XIV, protease XXIII and α-chymotrypsin type II were studied in this work. The degradation behaviors were expressed by their weight loss, morphological and secondary structure changes as well as thermal properties. Samrong showed higher percentage of weight loss than Nanglai. SEM micrographs indicated that silk fibroin were de- stroyed and showed many holes on their fiber surfaces. All of silk samples were increasable destroyed when exposure to the protease enzyme for long incubation period. With thermal analysis, both silk fibroin presented the thermal stability in the same profile. The result suggested that the selected silk fibroin should be composed of similar pattern of amino acids and their ratios. However, the protease susceptibility of each silk fibroin slightly varied in case of morphology observation. This might be affected by their genetic variety. Keywords: Degradation; Enzyme; Protease; Samrong; Nanglai 1. Introduction [10]. The degradation rate of silk may be highly variable depending on its structural and morphological features as well as characteristics of the biological environment. Previous literatures have been investigated the degra- dation behaviors of silk fibroins exposed to different proteolytic enzymes such as protease XIV [11], α-chy- motrypsin [12], protease XXI [13]. Separ