Dynamic changes in enzyme activities and phenolic content during in vitro rooting of tree peony (Paeonia suffruticosa Andr.) plantlets.docVIP

Maejo Int. J. Sci. Technol. 2011, 5(02), 252-265 Maejo International Journal of Science and Technology ISSN 1905-7873 Available online at www.mijst.mju.ac.th Full Paper Dynamic changes in enzyme activities and phenolic content during in vitro rooting of tree peony (Paeonia suffruticosa Andr.) plantlets Zhenzhu Fu , Panpan Xu , Songlin He , Jaime A. Teixeira da Silva and Michio Tanaka 1 1 1,* 2 2 1 Faculty of Forestry, Henan Agricultural University, No.95 Wenhua Road, Zhengzhou 450002, China 2 Department of Horticultural Science, Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho, Ikenobe 2393, Kagawa-ken 761-0795, Japan * Corresponding author, e-mail: hsl213@163.com Received: 15 April 2011 / Accepted: 4 July 2011 / Published: 28 July 2011 Abstract: The dynamic changes of phenolic content and peroxidase (POD), polyphenol oxidase (PPO), indole-3-acetic acid oxidase (IAAO) and phenylalanine ammonia lyase (PAL) activities were assessed during the in vitro rooting process of three cultivars of tree peony (Paeonia suffruticosa Andr.). These changes in enzyme-related activity and phenolic content observed at the level of the whole plant differed during the first 20 days __ __ of the rooting process in easy-to-root ‘Feng Dan Bai’ cultivar and difficult-to-root ‘Wu Long Peng Sheng’ and ‘Tai Ping Hong’ cultivars, and in most cases they were actually opposite. The ease with which ‘Feng Dan Bai’ was able to root was closely related to the activity of all four enzymes (POD, PPO, IAAO, PAL) as well as to the phenolic content. Keywords: tree peony, Paeonia suffruticosa, rooti