Short Term Outcome of Human Fetal Ovarian Tissue Cryopreservation and Xenotransplantation to Immunodefficient Mice..pdfVIP

ABSTRACT This study was designed to evaluate follicular survival an growth after short-term transplantation of cryopreserved and fresh human fetal ovarian tissue to immunodefficient mice. Two sets of transplantation were performed. A total of nine mice of strain Balb/c-nu divided into two groups- GROUP 1 and GROUP 2. Group 1 consist of five mice and group 2 consist of four mice .The first set of transplantation was done on the group 1 mice and all five nude mice in the group were subcutaneously transplanted with frozen – thawed human fetal ovarian tissue . The second set of transplantation was done on group 2 mice and all four mice were transplanted with fresh ovarian tissue. Two weeks after the subcutaneous transplantation of the ovarian tissue into the nude mice , the animals were killed and the grafted tissue removed .The extent of graft survival , as well as its histological and morphological changes were analysed by microscopy after Hematoxylin and Eosin Staining. Human adult and fetal ovarian tissue were also compared by immunohistochemistry staining using anti-HLA-DR antibody for the extent of expression of the Major Histocompatibility Complex (MHC) class II antigen HLA-DR. Our results demonstrate human fetal ovarian follicular survival after the two –week grafting period in the mice transplanted with fresh ovarian tissue ; extensive necrosis and fibrosis in the cryopreserved transplanted tissues. The expression of HLA-DR was higher in the adult ovarian tissue than the fetal ovarian tissue. In conclusion , there is successful follicular surv