Anti-Tumor Activity of Eurycoma longifolia Root Extracts against K-562 Cell Line In Vitro and In Vivo Study.docVIP

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Anti-Tumor Activity of Eurycoma longifolia Root Extracts against K-562 Cell Line In Vitro and In Vivo Study.doc

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Anti-Tumor Activity of Eurycoma longifolia Root Extracts against K-562 Cell Line In Vitro and In Vivo Study

Anti-TumorActivityofEurycomalongifoliaRootExtracts againstK-562CellLine:InVitroandInVivoStudy OmarSaeedAliAl-Salahi1.,DanJi2.,AminMalikShahAbdulMajid3,ChanKit-Lam3, WanZaidahAbdullah4,AbdelhamidZaki1,5,ShahKamalKhanJamalDin6,NarazahMohdYusoff1*, AmanShahAbdulMajid1*. 1AdvancedMedicalandDentalInstitute(AMDI),UniversitiSainsMalaysia(USM),KepalaBatas,PulauPinang,Malaysia,2KeyLabofVisualDamageandRegeneration RestorationofChongqing,SouthwestEyeHospital,SouthwestHospital,TheThirdMilitaryMedicalUniversity,Chongqing,P.R.China,3SchoolofPharmaceuticalSciences, UniversitiSainsMalaysia,Penang,Malaysia,4HaematologyDepartment,SchoolofMedicalSciences,USM,KubangKerian,Kelantan,Malaysia,5TherapeuticChemistry Department,NationalResearchCentre,CairoUniversity,Dokki,Cairo,Egypt,6OMFSurgery,HospitalSultanahBhiyah,AlorSetar,Kedah,Malaysia Abstract Eurycoma longifolia Jack has been widely used in traditional medicine for its antimalarial, aphrodisiac, anti-diabetic, antimicrobial and anti-pyretic activities. Its anticancer activity has also been recently reported on different solid tumors, howevernoanti-leukemicactivityofthisplanthasbeenreported.Thusthepresentstudyassessestheinvitroandinvivo anti-proliferativeandapoptoticpotentialsofE.longifoliaonK-562leukemiccellline.TheK-562cells(purchasedfromATCC) wereisolatedfrompatientswithchronicmyelocyticleukemia(CML)weretreatedwiththevariousfractions(TAF273,F3and F4) of E. longifolia root methanolic extract at various concentrations and time intervals and the anti-proliferative activity assessed by MTS assay. Flow cytometry was used to assess the apoptosis and cell cycle arrest. Nude mice injected subcutaneously with 107K-562 cells were used to study the anti-leukemic activity of TAF273 in vivo. TAF273, F3 and F4 showed various degrees of growth inhibition with IC50 values of 19, 55 and 62mg/ml, respectively. TAF273 induced apoptosis in a dose and time dependent manner. TAF273 arrested cell cycle at G1and S phases. Intraperitoneal administration