Apoptosis Signal-Regulating Kinase 1 Is Involved in WISP-1–Promoted Cell Motility in Human Oral Squamous Cell Carcinoma Cells.docVIP

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Apoptosis Signal-Regulating Kinase 1 Is Involved in WISP-1–Promoted Cell Motility in Human Oral Squamous Cell Carcinoma Cells.doc

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Apoptosis Signal-Regulating Kinase 1 Is Involved in WISP-1–Promoted Cell Motility in Human Oral Squamous Cell Carcinoma Cells

ApoptosisSignal-RegulatingKinase1IsInvolvedin WISP-1–PromotedCellMotilityinHumanOralSquamous CellCarcinomaCells Jing-YuanChuang1,An-ChenChang1,I-PingChiang2,Ming-HsuiTsai3.,Chih-HsinTang 4,5,6 . * 1Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan, 2Department of Pathology, China Medical University Hospital,Taichung,Taiwan,3DepartmentofOtolaryngology,ChinaMedicalUniversityHospital,Taichung,Taiwan,4GraduateInstituteofBasicMedicalScience,China MedicalUniversity,Taichung,Taiwan,5DepartmentofPharmacology,SchoolofMedicine,ChinaMedicalUniversity,Taichung,Taiwan,6DepartmentofBiotechnology, CollegeofHealthScience,AsiaUniversity,Taichung,Taiwan Abstract Oral squamous cell carcinoma (OSCC) has a tendency to migrate and metastasize. WNT1-inducible signaling pathway protein1(WISP-1)isacysteine-richproteinthatbelongstotheCyr61,CTGF,Nov(CCN)familyofmatrixcellularproteins. TheeffectofWISP-1onhumanOSCCcells,however,isunknown.Here,weshowedthatWISP-1increasedcellmigrationand intercellularadhesionmolecule-1(ICAM-1)expressioninOSCCcells.Pretreatmentofcellswithintegrinavb 3monoclonal antibody(mAb)significantlyabolishedWISP-1–inducedcellmigrationandICAM-1expression.Ontheotherhand,WISP-1– mediatedcellmotilityandICAM-1upregulationwereattenuatedbyASK1,JNK,andp38inhibitor.Furthermore,WISP-1also enhancedactivatorprotein1(AP-1)activation,andtheintegrinavb3mAb,andASK1,JNK,andp38inhibitorsreducedWISP- 1–mediated AP-1 activation. Moreover, WISP-1 and ICAM-1 expression correlated with the tumor stage of patients with OSCC.OurresultsindicatethatWISP-1enhancesthemigrationofOSCCcellsbyincreasingICAM-1expressionthroughthe avb3integrinreceptorandtheASK1,JNK/p38,andAP-1signaltransductionpathways. Citation:ChuangJ-Y,ChangA-C,ChiangI-P,TsaiM-H,TangC-H(2013)ApoptosisSignal-RegulatingKinase1IsInvolvedinWISP-1–PromotedCellMotilityin HumanOralSquamousCellCarcinomaCells.PLoSONE8(10):e78022.doi:10.1371/journal.pone.0078022 Editor:RajeshMohanraj,UAEUniversi