B7-H1 Expression Is Associated with Poor Prognosis in Colorectal Carcinoma and Regulates the Proliferation and Invasion of HCT116 Colorectal Cancer Cells.docVIP

B7-H1 Expression Is Associated with Poor Prognosis in Colorectal Carcinoma and Regulates the Proliferation and Invasion of HCT116 Colorectal Cancer Cells , Ming Wei , Yan-Ling Meng , An- Sheng-Jia Shi1?, Li-Juan Wang2?, Guo-Dong Wang3,4?, Zhang-Yan Guo1 3 1 Gang Yang , Wei-Hong Wen 1* 1* 1 State Key Laboratory of Cancer Biology, Department of Immunology, Fourth Military Medical University, Xi’an, China, 2 Department of Oncology, the First Affiliated Hospital, College of Medicine, Xi’an Jiaotong University, Xi’an, China, 3 Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi’an, China, 4 Department of Comprehensive Medicine, 323 Hospital of the Chinese People’s Liberation Army, Xi’an, China Abstract Background And Objective: The investigation concerning the B7-H1 expression in colorectal cancer cells is at an early stage. It is unclear whether B7-H1 expression may have diagnostic or prognostic value in colorectal carcinoma. Additionally, how B7-H1 is associated with the clinical features of colorectal carcinoma is not known. In order to investigate the relationship between B7-H1 and colorectal cancer, we analyzed B7-H1 expression and its effect in clinical specimens and HCT116 cells. Methods: Paraffin-embedded specimens from 143 eligible patients were used to investigate the expression of CD274 by immunohistochemistry. We also examined whether B7-H1 itself may be related to cell proliferation, apoptosis, migration and invasion in colon cancer HCT116 cells. Results: Our results show that B7-H1 was highly expressed in colorectal carcinoma and was significantly associated with cell differentiation status and TNM (Tumor Node Metastasis) stage. Patients with positive B7-H1 expression showed a trend of shorter survival time. Using multivariate analysis, w