Choline Plasmalogens Isolated from Swine Liver Inhibit Hepatoma Cell Proleration Associated with Caveolin-1Akt Signaling.docVIP

Choline Plasmalogens Isolated from Swine Liver Inhibit Hepatoma Cell Proliferation Associated with Caveolin-1/Akt Signaling Yaoyao Zhan , Liang Wang , Jing Liu , Keli Ma , Cuiping Liu , Yuan Zhang , Wei Zou 1,3 1 2 4 1 1,5 1* 1 College of Life Science, Liaoning Normal University, Dalian, Liaoning, P. R. China, 2 Regenerative Medicine Center, the First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, P.R.China, 3 College of Fisheries and Life Science, Dalian Ocean University, Dalian, Liaoning, P. R. China, 4 Department of Biochemistry and Molecular Biology, Dalian medical University, Dalian, Liaoning, P. R. China, 5 Key Laboratory of Molecular Enzymology, the Ministration of Education, Jilin University, Changchun, Jilin, P. R. China Abstract Plasmalogens play multiple roles in the structures of biological membranes, cell membrane lipid homeostasis and human diseases. We report the isolation and identification of choline plasmalogens (ChoPlas) from swine liver by high performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC)/MS. The growth and viability of hepatoma cells (CBRH7919, HepG2 and SMMC7721) was determined following ChoPlas treatment comparing with that of human normal immortal cell lines (HL7702). Result indicated that ChoPlas inhibited hepatoma cell proliferation with an optimal concentration and time of 25 μmol/L and 24 h. To better understand the mechanism of the ChoPlas-induced inhibition of hepatoma cell proliferation, Caveolin-1 and PI3K/Akt pathway signals, including total Akt, phospho-Akt(pAkt) and Bcl-2 expression in CBRH7919 cells, were determined by western blot. ChoPlas treatment increased Caveolin-1 expression and reduced the expression of