Vitrification in Open and Closed Carriers at Different Cell Stages Assessment of Embryo Survival, Development, DNA Integrity and Stability during Vapor Phase Storage for Transport 英文论文文献.docVIP
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Vitrification in Open and Closed Carriers at Different Cell Stages Assessment of Embryo Survival, Development, DNA Integrity and Stability during Vapor Phase Storage for Transport 英文论文文献
AbdelHafezetal.BMCBiotechnology2011,11:29
/1472-6750/11/29
RESEARCH ARTICLE
OpenAccess
VitrificationinOpenandClosedCarriersat
DifferentCellStages:AssessmentofEmbryo
Survival,Development,DNAIntegrityand
StabilityduringVaporPhaseStoragefor
Transport
FatenAbdelHafez,JingXu,JeffreyGoldbergandNinaDesai*
Abstract
Background:Highcoolingrateswithvitrificationcanbeachievedthroughtheuseofcarriersthatallow
cryopreservationinfluidvolumesoneμl.Opencarriersallowdirectcontactofembryoswithliquidnitrogen
(LN2)whereasclosedcarriersystemssequestertheembryowithinasealedsystemduringimmersioninLN2.The
useofclose
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