effects of ve and vc on reactive xoxgen species-related lead toxicity.pdfVIP

Toxicology 128 (1998) 169–179 Effects of vitamin E andor C on reactive oxygen species-related lead toxicity in the rat sperm Ping-Chi Hsu a,b, Ming-Yie Liu a, Chao-Chin Hsu c, Lih-Yuh Chen d, Yueliang Leon Guo a,* a Department of Enironmental and Occupational Health, National Cheng Kung Uniersity Medical College, 61 Shiao Tong Road, Tainan 70428, Taiwan, ROC b Department of Industrial Safety and Hygiene, Chai-Nan College of Pharmacy and Science, Tainan 70428, Taiwan, ROC c Department of Obstetrics and Gynecology, National Cheng Kung Uniersity Medical College, 61 Shiao Tong Road, Tainan 70428, Taiwan, ROC d Department of Physiology, National Cheng Kung Uniersity Medical College, 61 Shiao Tong Road, Tainan 70428, Taiwan, ROC Received 8 January 1998; accepted 7 May 1998 Abstract This study was undertaken to investigate whether treatment with vitamin E (VE) andor vitamin C (VC) protects rat sperm by inhibiting reactive oxygen species generation induced by lead (Pb) exposure. Male Sprague–Dawley rats were assigned to the following five groups: vitamin-unsupplemented; 150 mg VEkg chow supplemented; 300 mg VEkg chow supplemented; 500 mg VCl drinking water supplemented and 150 mg VEkg chow 500 mg VCl drinking water supplemented group. Rats in each group were divided into Pb-unexposed and Pb-exposed subgroups, received weekly intraperitoneal injection of 10 mg sodium acetate or 10 mg Pb acetatekg for 6 weeks, respectively. The blood and sperm Pb levels were analyzed by graphite furnace atomic absorption spectrophotometer. Chemilu- minescence was