杆菌基因克隆表达及其抗原表位分析.pdf

ChinJPublicHealthV01．24No．9 1090 中国公共卫生2008年9月第24卷第9期 Sep2008 文章编号：1001—0580(2008}09．1090．04中圉分类号：R392．11文献标志码：A 【论 著】 幽门螺杆菌基因克隆表达及其抗原表位分析* 胡平，罗军，赵卫，张文炳，龙北国 构建HP0410抗原表位的抗原展示库。竞争一抑制实验验证获得的阳性噬菌体并测序，生物信息学分析HP0410的抗 原表位。结果成功构建可高水平分泌型表达HP0410的原核表达系统。对13株阳性噬菌体测序后获得8个表位， HP0410同源性不高，但处于生物信息学预测的区域。绪论获得8个HP0410高抗原性区域的模拟表位，其中1个 为模拟单抗E018特异性结合的抗原决定基的候选表位。 关键词：HP0410；12肽展示库；生物信息学 Cloneand ofHelicobacter HP0410andits W矗，et expression pyloriprotein epitopes H【，Ping。LUOJun。ZHAO analysis a1．Department ofMicrobiology，School 510515，China) To the of of Abstract：0bjectivefeature HP0410—8surface ofHelicobacterbyusing study epitopes protein pylori used phage technology．MethodsWefirstconstructedan ofHP0410inE．coliB1．21andthenwe display expressionsystem the recombinantscan and purified to monoelonalantibodiesof one monoelonal protein Hp got specific wasused tOconstructa M13 ofHP0410．TheDNAof was and antibody 12一peptidesphagelibrary positivephagesequenced thecandidatewast铬ted were bioinformaticsmethods．Results by competitive—inhibitionarray．Theepitopesanalyzedby Weobtained8 andacandidatefromthe 13 DNAand ofbioinfor— epitopes epitope phagelibrarybysequencingphages analysis matics．The HP0410could the betweencandidateand competiti