小鹅瘟羊源高免血清的制备及比较.pdfVIP

资州农业科学 2009 ， 37(10):127~129 Guizhou Agricultural Sciences [文章编每]1001-3601(2009)10心708-0121心3 小鹅瘟羊源高兔血清的制备及比较 ~文强1 ，革开功2· ，回新平z (1.铜仁职业技术学院，贵州铜仁 55430(. 2. 贵州大学动物科学学院，贵阳花溪 550025 ， 3. 锅仁市畜牧兽医局，贵州锅仁 554300) [摘 要〕为制备高效价的小鸦痕高免血清，试验来用从贵州某养鹅场病鹅分窝的小鹅痰野生强毒株，接 种于 9 d 鹅脏，收集 24 h 以后死亡的鹅腮尿囊液，制备小鹅痕病毒抗尿，与弗氏佐剂混合均匀，多次免疫山 羊后，颈动脉一次性放血，离心收集血清即得到小鹅瘟羊源高先血清，以琼脂扩散地行血清效价测定，并与市 售小鹅瘟鹅源高兔血清进行保护率比较。结果显示，试验制备的小鹅瘟羊派高兔血清抗体效价在 1 : 8 以 上，对人工感染雏鹅的保护牟为 95% ，而市售小鹅瘟鹅派高免血清的保护卒只有 85%. 结果在明，本试验制 备的小鸪瘟羊源离免血清具有效价高、成本低、安全性好等优点。 [共键词]小鹅瘟g 羊派高克血清;和i 备;比较 [中圈分提号] 5858.33 [文献标识码]A Preparation of High Immune Serum with Resistance to Goose pαrvovirus from Goat Source and Its Protection Rate WANG Wen-qiang1 , WANG Kai-gongZ* , TIAN Xin-ping3 (1, Tongren Vocational and Technical College , Tongren. Gui:.hou 554300; 2. College 01 Animal Sciences. Gui:.hou Vniversit,. Guiyang. Guix.hou 550025; 3. Animal Husbandry and Veterinary Bureau 01 Tongren City , Tongren. Gui:.hou 554300.ωina) Abstract: The antigen of goose parvirus was prepared from al1antotic f1 uids of goose embryos infected by the highly virulent strain isolated from a goose farm in Guizhou Province , The high immune serum with resistance to goose plague was obtained by centrifugally separating the blood from immune goats vaccinated by the míxed fluid of the antigen of goose parvirus and Freund 5 agent. The antibody titer of the high immune serum was determined by agar-gel precipitation and its antibody titer reached to 1 : 8 and its protection rate of artificial infected baby geese was up to 95 %, which índicates the high immune serum with resistance to goose parvirus has the advantages of higher antibody titer , lower cost and good safety. Key words: gosling