动物源乳杆菌筛选及生物特性参数测定.pdfVIP

( ) 江苏农学院学报 JOU RN A L O F J IA N GSU A GR ICUL TU RA L COL L EGE 1997, 18 1 : 1～ 5 动物源乳杆菌筛选及生物特性参数测定 王传彬　王永坤　朱国强　周继宏 (江苏农学院动物医学系, 扬州 225009) 摘　要　根据益生素选择的关键指标, 从健康动物肠道筛选到鸡源乳杆菌2 株, 猪源乳杆菌 3 株。其中嗜酸乳杆菌 3 株、 唾液乳杆菌 1 株、短乳杆菌 1 株。5 株乳杆菌均能耐受浓度为 0. 5% 苯酚和 0. 8%～ 1. 0% 牛胆盐, 对pH 3. 0 有较强的抵 抗性, 此 3 项指标均优于保加利亚乳杆菌。其代谢产物对常见致病菌均有一定程度的抑制作用。这 5 株乳杆菌对小白鼠 和 1 日龄雏鸡均无毒性作用。2 株鸡源乳杆菌对雏鸡小肠粘膜上皮细胞有良好的粘附性。 关键词　动物; 乳杆菌; 生物特性; 益生素 中图法分类号　 852. 612 S SCREEN ING AND STUDY ING ON B IOLOGICAL CHARACTER IST ICS OF LACTOBAC ILL I OF AN IM AL OR IGIN 　 　 　 W ang Chuanbin W ang Y ongkun Zhu Guoq iang Zhou J ihong ( . . . , . . , 225009) D ep t of V et M ed J iang su A g ric Coll Yangzhou 　 , ABSTRACT A cco rding to key p roperties required by p robio tics tw o lactobacillus strains of ch icken o rigin and th ree of p ig o rigin w ere iso lated and identified from anim al intestine respectively. O f the five lactobacilli, one L act. salivarius , . , . . strain one L act brevies strain and the o ther th ree w ere L act acidoph ilus A ll of the five L actobacilliw ere to lerant to 0. 8%～ 1. 0% bile salts and 0. 4% pheno l, and resistant to pH 3. 0. T he th ree p roperties of the five lactobacilli of anim al . . o rigin w ere better than L act bulgaricus T he m etabo lite of the five lactobacillus strains exh ibited antagonistic activity . . to