碱胁迫下PGPR菌株对水稻萌发及水稻幼苗生_省略_脱氨酶活性的PGPR在盐碱逆境.pdfVIP

, Journal of Anhu Agr . Sc . 2007, 35( 34) : 11060- 11062 刘月娟 俞洁 PGPR 具ACC 脱氨酶活性的PGPR 在盐碱逆境下的应用初探 赵骥民, 辛树权, 何正飚, 王贵, 章有知 ( , 130032) [ 的] 解决苏打型盐碱稻作区水稻在育秧阶段受盐碱危害而导致的种子发芽势低发芽不齐芽尖枯黄弯曲, 幼苗生长不良甚 至死亡的难题[ 方法] 用具ACC 脱氨酶活性的PGPR 菌株处理水稻种子, 在不同浓度的碳酸钠( 碳酸氢钠) 溶液的碱胁迫下进行水稻种 子的萌发实验和幼苗的生长试验, 测定水稻种子的发芽势发芽率, 水稻幼苗的根长苗高及不定根数[ 结果] 结果表明: 水稻种子用菌 株处理后, 发芽势和发芽率都显著地提高, 在碱浓度为1. 25% 时水稻种子的发芽势均超过40% , 发芽率在80% 以上; 同时, 该处理也有效 地拓宽了水稻幼苗在碱胁迫下生存的生态幅, 在1.25% 的碳酸钠浓度下, 水稻种子能萌发并继续生长, 而此浓度下未用菌株处理的水稻 种子却不能萌发; 在各浓度的碱胁迫下, 水稻处理组的幼苗根长苗长和不定根的数 均显著地优于对照组[ 结论] 具ACC 脱氨酶活性 的PGPR 能够有效地解决水稻种子在盐碱胁迫下萌发及其幼苗生长阶段所受到的危害 ACC 脱氨酶; 碱胁迫; 发芽势;根长; 苗高 S 182 A 0517- 6611( 2007) 34- 11060- 03 Promoting Effect of Two PGPR Strains on the Rice Germination and SeedlingsGrowth undertheAlkali Stress ZHAOJ-i min et al ( College of L fe Sc ence, Changchun Normal Un vers ty, Changchun, J l n 130032) Abstract [ Object ve] In order to solve the d ff cult problems wh ch resulted from the salt-alkalo d harmfulness n salt-alkal so l r ce area, nclud ng low r ce germ nat on v gor, no neat germ nat on, the w thered and curved bud t p, and the bad growth even dead. [Method] The seeds were treated by PGPR stra ns w th act v ty of ACC deam nase to conduct the r ce seedl ng germ nat on exper ment and the seedl ngs growth exper ment under the stress of alkal w th dfferent concentrat ons, and the germ nat on v gor, germ nat on rate of r ce seeds , the root length, seedl ng he ght and lateral root numbers of r ce seedl ngs were also measured. [Result] After treat ng r ce w th PGPR stra ns the germ nat on v gor and germ nat on rate s gn f cantly mproved, the germ- nat on v gor of r ce seeds was over 40% and the germ nat on rate was more than 80% under alkalo d concentrat on of 1. 25% . The treatment eff ect vely w dened the ecolog cal ampl tude of r ce seedl ngs surv ved n the stress of the alkal . The r ce seed could germ nate and grow at 1. 2