Analysis of Urea in Petfood Matrices Comparison of Spectro-Colorimetric, Enzymatic and Liquid Chromatography Electrospray Ionization High Resolution Mass Spectrometry Methods英文文献资料.docVIP

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Analysis of Urea in Petfood Matrices Comparison of Spectro-Colorimetric, Enzymatic and Liquid Chromatography Electrospray Ionization High Resolution Mass Spectrometry Methods英文文献资料.doc

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American Journal of Analytical Chemistry, 2012, 3, 613-621 /10.4236/ajac.2012.39080 Published Online September 2012 (http://www.SciRP.org/journal/ajac) Analysis of Urea in Petfood Matrices: Comparison of Spectro-Colorimetric, Enzymatic and Liquid Chromatography Electrospray Ionization High Resolution Mass Spectrometry Methods Patrick Pibarot , Serge Pilard 1 2 Nestlé Research and Development Centers, Aubigny, France 1 2 Analytical Platform, University of Picardie Jules Verne, Amiens, France Email: Patrick.Pibarot@ Received July 4, 2012; revised August 12, 2012; accepted August 19, 2012 ABSTRACT Adulteration may consist in non authorized source of nitrogen addition to increase the protein content of some raw ma- terials. Urea which is authorized for feed is a non nutritional source of nitrogen in food and pet food. Adulteration of food or pet food raw material by urea is thus monitored by manufacturer and governmental authorities with official methods which are either enzymatic (Association of Official Agricultural Chemists, AOAC) or spectro-colorimetric (European Community, EC). Each method gives results which are not comparable and spectro-colorimetric methods may result in false-positive urea detection. Liquid chromatographic (LC/UV-DAD) analysis of extracts from spectro- colorimetric method indicates that presence of free amino-acid may interfere with colorimetric detection of urea in the EC method with pet food samples. Liquid chromatography electrospray ionization high resolution mass spectrometry (LC/ESI-HRMS) has allowed to quantify low content (0.01%) of urea in pet food water extracts for samples which resulted in significant urea detection with colorimetric method and in content below the detection threshold with enzy- matic method. This study demonstrates the EC colorimetric method is not applicable to pet food and also food samples which have a complex compositi