Annexin 1 localisation in tissue eosinophils as detected by electron microscopy英文文献资料.docVIP

Research Communication Mediators of Inflammation, 11, 287–292 (2002) B ACKGROUND: Human and rodent leukocytes express Annexin 1 localisation in tissue eosinophils as detected by electron microscopy high levels of the glucocorticoid-inducible protein annexin 1 (ANXA1) (previously referred to as lipo- cortin 1). Neutrophils and monocytes have abundant ANXA1 levels. Aim: We have investigated, for the first time, ANXA1 ultrastructural expression in rat eosinophils and compared it with that of extravasated neutrophils. The effect of inflammation (carrageenin peritonitis) was also monitored. Sonia M. Oliani , Amilcar S. Damazo and Mauro 1,2 2 Perretti 3,CA Methods: Electron microscopy was used to define the sub-cellular localisation of ANXA1 in rat eosinophils and neutrophils extravasated in the mesenteric tis- sue. A pair of antibodies raised against the ANXA1 N-terminus (i.e. able to recognise intact ANXA1, termed LCPS1) or the whole protein (termed LCS3) was used to perform the ultrastructural analysis. Results: The majority of ANXA1 was localised in the eosinophil cytosol (~60%) and nucleus (30–40%), whereas a small percentage was found on the plasma membrane ( 10%). Within the cytosol, the protein was equally distributed in the matrix and in the granules,includingthosecontainingthetypicalcrys- talloid. The two anti-ANXA1 antibodies gave similar results, with the exception that LCPS1 gave a lower degree of immunoreactivity in the plasma membrane. Inflammation (i.e. carrageenin injection) produced a modest increase in eosinophil-associated ANXA1 reactivity (significant only in the cytoplasm compart- ment). Extravasated neutrophils, used for compar- ative purposes, displayed a much higher degree of immunoreactivity for the protein. 1 Department of Biology, IBILCE – UNESP, Sa?o Jose′ 2 do Rio Preto, SP, Brazil; Department of Morphology, 3 UNIFESP – EPM, Sa?o Paulo, SP, Brazil; and The William Harvey Res