Apelin-12 improves metabolic and functional recovery of rat heart after global ischemia英文文献资料.docVIP

Vol.2, No.8, 927-934 (2010) HEALTH doi:10.4236/health.2010.28137 Apelin-12 improves metabolic and functional recovery of rat heart after global ischemia Oleg I. Pisarenko*, Valentin S. Shulzhenko, Yulia A. Pelogeykina, Irina M. Studneva, Denis N. Khatri Russian Cardiology Research-and-Production Complex, Moscow, Russia; *Corresponding Author: olpi@cardio.ru Received 27 March 2010; revised 24 April 2010; accepted 26 April 2010. ABSTRACT in energy metabolism may play a role in me- chanisms of cardioprotection afforded by A-12 during I/R stress. This work was designed to explore efficacy of apelin-12 (A-12) as a cardioprotective agent when given before ischemia or at reperfusion using the isolated working heart model. Hearts of male Wistar rats were subjected to 30-min stabilization period followed by 35-min global ischemia and 30-min reperfusion. A short-term infusion of Krebs-Henseleit buffer (KHB) con- taining A-12 (35, 70, 140, 280 or 560 ?M) was ap- plied prior to ischemia (A-12-I) or at onset of reperfusion (A-12-R). KHB infusion was used as control. A-12 infusions induced a dose-depen- dent increase in recovery of coronary flow, contractile and pump function during reperfu- sion, with the largest augmentation of these indices in the A-12-I group. Both A-12 groups exhibited a significant reduction of LV diastolic pressure rise during reperfusion compared with control. Enhanced functional recovery in the A-12-I group was combined with a decrease in LDH leakage in perfusate on early reperfusion (by 36% vs. control, p 0.05). Preischemic infu- sion of 140 ?M A-12 markedly increased myo- cardial ATP content, enhanced preservation of the total adenine nucleotide pool and improved recovery of the energy charge in reperfused hearts. There was a trend towards increase in myocardial phosphocreatine by the end of re- perfusion in the A-12