Ca (II)-EDTA shows antimicrobial activity against periodontopathic bacteria英文文献资料.docVIP

J. Biomedical Science and Engineering, 2012, 5, 10-14 JBiSE /10.4236/jbise.2012.51002 Published Online January 2012 (http://www.SciRP.org/journal/jbise/) Ca (II)-EDTA shows antimicrobial activity against periodontopathic bacteria Tadashi Miura, Koji Tanabe, Masao Yoshinari Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College, Chiba, Japan Email: tamiura@tdc.ac.jp Received 18 October 2011; revised 28 November 2011; accepted 7 December 2011 ABSTRACT are to be prevented. Research at our laboratory has fo- cused on developing a system of defense against infec- tion on dental material surfaces [5]. Decreasing plaque accumulation has been emphasized in the prevention of periodontal disease, including peri-implantitis [6]. An- timicrobial materials may offer a means of reducing the oral bacteria involved in the development of oral disease. In an earlier study, we found that one advantage of an- timicrobial peptides was that they did not act as antigens against the host [7]. One of the aims of our ongoing re- search is to create a defense system against peri-implan- titis, and we have recently been exploring the antimicro- bial potential of low-molecular materials affixed to the surfaces of dental materials [8]. We have reported the anti-periodontopathic activity of the antimicrobial pep- tide protamine [9]. Although protamine has shown strong inhibitory activity against growth of P. gingivalis, its antimicrobial force appears to be short-lived, and prob- lems remain with regard to its sustained release. There- fore, we are searching for other low-molecular sub- stances which can exert a more stable antibacterial effect. In the present study, we investigated the inhibitory ef- fect of ethylenediamine-N,N,N’,N’-tetraacetic acid cal- cium (II) disodium salt (Ca (II)-EDTA) on growth of periodontopathic bacteria. Ethylenediaminetetraacetic acid (EDTA) in