S-腺苷-L-蛋氨酸高密度发酵工艺优化.PDFVIP

生 物 工 程 学 报 Chin J Biotech 2009, April 25; 25(4): 533-536 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn © 2009 Institute of Microbiology, CAS CSM, All rights reserved 工业生物技术 S--L- 王杰鹏, 韩晋军, 李晓楠, 刘沛溢, 谭天伟 , 100029 : 利用酿酒酵母在 5 L 发酵罐高密度发酵生产 S-腺苷-L-蛋氨酸(SAM)后期存在稳定性差的问题, 本研究考察了 在补糖中添加磷酸氢二铵、谷氨酸钠、三磷酸腺苷二钠来提高酿酒酵母发酵后期的稳定性。通过 4 批 5 L 发酵罐高密 度流加发酵实验研究发现: 在发酵 34 h 左右, 菌体干重超过 100 g/L 后, 开始添加 50 克 L-蛋氨酸, 并在补糖中加入 10 g/L 三磷酸腺苷二钠, 发酵 65.7 h, 最高生物量干重达到 180 g/L, SAM 产量达到 17.1 g/L。 : S-腺苷-L-蛋氨酸, ATP, 高密度发酵, L-蛋氨酸 Optimization of high-cell-density fermentation process for S-adenosyl-L-methionine production Jiepeng Wang, Jinjun Han, Xiaonan Li, Peiyi Liu, and Tianwei Tan College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China Abstract: Poor stability existed in the anaphase of the high-cell-density fermentation of Saccharomyces crevisiae for S-adenosyl-L- methionine (SAM) production in 5 L fermentor. To improve the fermentation stability, we studied the addition of diammonium hydrogen phosphate, sodium glutamate and adenosine disodium triphosphate into glucose feeding solution. Study of four fed-batch cultures showed that, after 34 h fermentation, when dry cell weight reached 100 g/L, the addition of 50 g pre-L-methionine and glucose feeding with 10 g/L adenosine disodium triphosphate was optimal for SAM production. Under this condition, after 65.7 h fermentation, both th