兰花炭疽病拮抗细菌5A5-3菌株的初步筛选和鉴定.pdfVIP

林业科学研究 2013 ， 26(5) :598 ~602 Forest Research 文章编号:1∞1-1498 (2013 )05 -0598 -05 兰花炭瘟病拮抗细菌5A5-3 菌株的 初步筛选和鉴定 曹晓璐1 ，刘慧娟2 ，郭晓军2 ，姚 娜1 ，李璐滨1 * (1.中国林业科学研究院林业研究所，林木遗传育种国家重点实验室，北京 1αlO91 ; 2. 河北农业大学生命科学学院，河北保定。71∞1) 摘要:从毛乌素沙地采集的 10 份土样中共分离到细菌320 株，将分离到的细菌菌株与兰花炭瘟病病原菌脏胞炭瘟 菌进行生长对峙试验。初筛获得拮抗菌57 株。复筛后得到筛选自毛乌素沙地土壤生物结皮样品的 5 A5-3 对病原 菌具有较高的抑菌活性。对其进行形态特征观察和生理生化试验及 16S rDNA 序列相似性分析，结果表明:茵株 5A5-3 与 Bacülω velezensis 标准菌株(CRδ02 ， AYω3658)， 的 16S rDNA 序列相似度达 1∞9岛，最终将菌株 5A5-3 鉴定 为 Bacillus veleze旧白。 关键词:兰花病害;拮抗细菌;筛选;鉴定 中固分类号:S718.8 文献标识码:A Preliminary Isolation and Identification of the Antagonistic Bacterial Strain 5A5 -3 Against Common Orchid Diseases 1 2 2 1 1 CAO Xiω_lu ， UU Hui-juan , GUO Xiω_jun ， YAO Na , U Lu-bin (1. Research Institute of Forest巧， Chinese Academy of Forestry , State Key Laboratory of Tree Genetics and Breeding , Beijing 1 ∞091 ， China; 2. Coll咿of Life Sciences , Agricultural University of Hebei , Baoding 071∞1 ， Hebei , China) Abstract: 320 strains were isolated from 10 soil samples col1ected at Mu Us sandy land of China. Mter growing confrontation test to Colletotrichum gloeosporioide , 57 strains were screened preliminarily. The strain 5A5 -3 screened from biological crusts of Mu Us sandy land with a rather higher antagonistic activity 吨;ainst C. gloeosporioide was ob- tained after secondary screening. The strain 5 A5 -3 w幽 further identified by morphological and cultural characteris- tics , physiological and biochemical experiments and 16S rDNA sequence analysis. Based on the results , the str