玉米秸秆、菇渣用于双孢蘑菇堆肥的-农业工程学报.doc

玉米秸秆、菇渣用于双孢蘑菇堆肥的 理化性状和细菌群落分析 秦改娟1, 郭亚萍1, 张国庆2, 陈青君1**, （1北京农学院植物科学技术学院农业应用新技术北京市重点实验室，北京 102206 2北京农学院生物科学与工程学院农业部都市农业（北方）重点实验室，北京 102206） 摘 要：研究目的：本研究以双孢蘑菇（Agaricus bisporus）传统麦草配方作为对照，分别用玉米秸秆和杏鲍菇菇渣替换部分麦草设计两个新配方，通过细菌群落动态及理化性状分析，评价玉米秸秆或杏鲍菇菇渣用于工厂化双孢菇生产的可行性。方法：分别在建堆、一次发酵结束、二次发酵结束3个时期采集堆肥样品，提取总DNA，以大肠杆菌（Escherichia coli）16S rDNA V3区通用引物，进行PCR‐DGGE 扩增和序列分析，同时分析培养料理化性状。结果：玉米秸配方基质颗粒比较粗大，含氮量较低，二次发酵结束时与麦草配方具有较多的共有优势菌群，两者的细菌多样性指数变化规律也一致。菇渣配方基质颗粒比较细碎，含氮量较高，其堆肥个时期的优势细菌种类、多样性指数变化趋势与麦草配方明显不同。结论：传统麦草配方具有良好的理化性状和稳定的细菌群落，玉米秸秆、杏鲍菇菇渣代替部分麦草的配方仍需进一步优化。 关键词：双孢蘑菇；堆肥期；理化性状；细菌群落结构；PCR-DGGE 中图分类号：S646.9 文献标识码：A Physical-chemical Properties and Bacterial Community Analysis of Different Spent Mushroom Composts Made by Corn Stalks or Oyster Mushroom Residues Qin Gaijuan1，Guo Yaping1，Zhang Guoqing2，Chen Qingjun1**， Yang Kai1 （1College of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing 102206, China 2College of Biological Sciences and Engineering, Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206, China） Abstract:Using the traditional wheat straw formula as control, two test compost formulas were designed using corn stalks or oyster mushroom (Pleurotus eryngii) residues replacing part of the wheat straw, respectively. At the beginning of composting, the end of phase I, and phase II, compost samples were collected and total DNA were further obtained. Bacterial communities of each sample were analysed using Polymerase Chain Reaction-Denaturing gradient gel electrophoresis (PCR-DGGE) method based on 16S rDNA using the universal primers from Escherichia coli. The physical-chemical properties of the samples and the yield of the three formulations were also measured and analysed. The results showed that the corn stalks formula shared higher particle size and lower nitrogen content than those of the wheat straw formula.At the end