轻微肝性脑病患者血清代谢组研究-第三军医大学学报.doc

轻微肝性脑病患者血清中代谢物组的研究 齐素文1,戴勇1,欧阳昕2,彭武建2,蔡安季1,涂植光1( (400016 重庆，重庆医科大学教育部重点实验室，临床医学检验诊断实验室1；510632 广东深圳，暨南大学第二临床医学院深圳市人民医院 临床医学研究中心2) [提要] 目的 检测初步建立MHE诊断模型。方法 采用核磁共振（NMR）方法肝硬化患者（肝硬化组）、轻微肝性脑病患者（MHE组）清中的代谢物，应用主成分分析（PCA）和正交偏最小二乘法-判别分析(OPLS-DA)对NMR谱数据进行模式识别分析，并用临床标本验证代谢组模型诊断MHE的可行性。结果 健康对照与肝硬化患者（包括肝硬化组和MHE组患者）之间的区分明显模型的质量参数R2X=35.1%, Q2Y =0.902肝硬化组和MHE组标本模型质量参数R2X =30.4%, Q2Y =0.817与相比，肝硬化组和MHE组患者血清中葡萄糖、琥珀酸盐、柠檬酸盐等含量增高, 低密度脂蛋白极低密度脂蛋白、胆碱等含量减少；与肝硬化组相比，MHE组患者的血清中的谷氨酰胺、苯丙氨酸、丙酮增高，缬氨酸和异亮氨酸降低。模型标本。结论 基于1H NMR代谢组学技术可同时检测患者血清中多种代谢物质，有助于研究肝硬化和MHE的发病机制，可能为MHE的诊断提供一种新的手段。 [关键词] 代谢组学；轻微肝性脑病；模式识别分析 [中图分类号] R575.2 ［文献标识码］A Study on Metabolic Signature of Serum in Patients with Minimal Hepatic Encephalopathy Qi Suwen1, Dai Yong1, Ouyang Xin2, Peng Wujian2, Cai Anji1, Tu Zhiguang1 (1Key laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing, 410006; 2Center of Clinical medicine, Second Clinical Medical College, Jinan University, Guangdong, 510632) [ABSTRACT] Objective To investigate the metabonomics in the serum samples from minimal hepatic encephalopathy (MHE) patients and to establish a diagnostic model for MHE. Methods Nuclear magnetic resonance spectroscopy (NMR) was applied to analyze the serums metabonomics from 25 simple 1iver cirrhosis patients, 25 cirrhotic patients with minimal hepatic encephalopathy (MHE) and 30 healthy volunteer. Principal component analysis (PCA) and orthogonal partial least-squares-discriminant analysis (OPLS-DA) were employed to establish the pattern recognition analysis of NMR spectra. Clinical samples were used to evaluate the performance of the diagnostic model. Results The OPLS-DA models was capable of distinguishing cirrhotic patients from healthy controls and, with an R2X=35.1%, a Q2Y =0.902. The OPLS-DA models was capable of distinguishing patients with and without MHE, with an R2X =30.4%, Q2Y =0.817. In comparison with healthy volunteers，the results show