A Baculovirus Immediate-Early Gene, ie1, Promoter Drives Efficient Expression of a Transgene in Both Drosophila melanogaster and Bombyx mori 英文参考文献.docVIP

ABaculovirusImmediate-EarlyGene,ie1,Promoter DrivesEfficientExpressionofaTransgeneinBoth DrosophilamelanogasterandBombyxmori MikaMasumoto1,2,TakahiroOhde1,KunihiroShiomi3,ToshinobuYaginuma1,TeruyukiNiimi1* 1GraduateSchoolofBioagriculturalSciences,NagoyaUniversity,Chikusa,Nagoya,Japan,2DivisionofBiology,CollegeofLiberalArtsandSciences,KitasatoUniversity, Sagamihara,Kanagawa,Japan,3FacultyofTextileScienceandTechnology,ShinshuUniversity,Ueda,Nagano,Japan Abstract Manypromotershavebeenusedtodriveexpressionofheterologoustransgenesininsects.Onemajorobstacleinthestudy ofnon-modelinsectsisthedearthofuseful promotersforanalysisofgenefunction. Here,weinvestigatedwhetherthe promoteroftheimmediate-earlygene,ie1,fromtheBombyxmorinucleopolyhedrovirus(BmNPV)couldbeusedtodrive efficient transgene expression in a wide variety of insects. We used a piggyBac-based vector with a 3xP3-DsRed transformation marker to generate a reporter construct; this construct was used to determine the expression patterns drivenbytheBmNPVie1promoter;weperformedadetailedinvestigationofthepromoterintransgeneexpressionpattern in Drosophila melanogaster and in B. mori. Drosophila and Bombyx belong to different insect orders (Diptera and Lepidoptera,respectively);however,andtooursurprise,ie1promoter-drivenexpressionwasevidentinseveraltissues(e.g., prothoracicgland,midgut,andtracheole)inbothinsects.Furthermore,inbothspecies,theie1promoterdroveexpression of the reporter gene from a relatively early embryonic stage, and strong ubiquitous ie1 promoter-driven expression continued throughout the larval, pupal, and adult stages by surface observation. Therefore, we suggest that the ie1 promotercanbeusedasanefficientexpressiondriverinadiverserangeofinsectspecies. Citation: Masumoto M, Ohde T, Shiomi K, Yaginuma T, Niimi T (2012) A Baculovirus Immediate-Early Gene, ie1, Promoter Drives Efficient Expression of aTransgeneinBothDrosophilamelanogasterandBombyxmori.PLoSONE7(11):e49323.doi:10.1371/journal.pone.004