A 3D Model of the Membrane Protein Complex Formed by the White Spot Syndrome Virus Structural Proteins 英文参考文献.docVIP

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A 3D Model of the Membrane Protein Complex Formed by the White Spot Syndrome Virus Structural Proteins 英文参考文献.doc

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A 3D Model of the Membrane Protein Complex Formed by the White Spot Syndrome Virus Structural Proteins 英文参考文献

A3DModeloftheMembraneProteinComplexFormed bytheWhiteSpotSyndromeVirusStructuralProteins Yun-ShiangChang1*.,Wang-JingLiu2.,Cheng-ChungLee3.,Tsung-LuChou1,Yuan-TingLee1 ,Tz-Shian Wu1,Jiun-YanHuang2,Wei-TungHuang1,Tai-LinLee1,Guang-HsiungKou2,AndrewH.-J.Wang3* ,Chu- FangLo2* 1Department of Molecular Biotechnology, Da-Yeh University, Changhua, Taiwan, 2Institute of Zoology, National Taiwan University, Taipei, Taiwan, 3Institute of BiologicalChemistry,AcademiaSinica,Taipei,Taiwan Abstract Background:Outbreaksofwhitespotdiseasehavehadalargenegativeeconomicimpactonculturedshrimpworldwide. However,thepathogenesisofthecausativevirus,WSSV(whitspotsyndromevirus),isnotyetwellunderstood.WSSVisa largeenvelopedvirus.TheWSSVvirionhasthreestructurallayerssurroundingitscoreDNA:anouterenvelope,ategument andanucleocapsid.Inthisstudy,weinvestigatedtheprotein-proteininteractionsofthemajorWSSVstructuralproteins, includingseveralenvelopeandtegumentproteinsthatareknowntobeinvolvedintheinfectionprocess. PrincipalFindings:Inthepresentreport,weusedcoimmunoprecipitationandyeasttwo-hybridassaystoelucidateand/or confirmalltheinteractionsthatoccuramongtheWSSVstructural(envelopeandtegument)proteinsVP51A,VP19,VP24, VP26andVP28.WefoundthatVP51AinteracteddirectlynotonlywithVP26butalsowithVP19andVP24.VP51A,VP19and VP24werealsoshowntohaveanaffinityforself-interaction.Chemicalcross-linkingassaysshowedthatthesethreeself- interactingproteinscouldoccurasdimers. Conclusions: From our present results in conjunction with other previously established interactions we construct a 3D modelinwhichVP24actsasacoreproteinthatdirectlyassociateswithVP26,VP28,VP38A,VP51AandWSV010toforma membrane-associatedproteincomplex.VP19andVP37areattachedtothiscomplexviaassociationwithVP51AandVP28, respectively.ThroughtheVP26-VP51Cinteractionthisenvelopecomplexisanchoredtothenucleocapsid,whichismadeof layersofringsformedbyVP664.A3Dmodelofthenucleocapsidandthesurroundingoutermembraneispresented. Citation:ChangY-S,LiuW-J,LeeC-C,ChouT