A Dynamic View of Domain-Motif Interactions 英文参考文献.docVIP

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A Dynamic View of Domain-Motif Interactions 英文参考文献.doc

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A Dynamic View of Domain-Motif Interactions 英文参考文献

ADynamicViewofDomain-MotifInteractions EyalAkiva¤a,GilgiFriedlander¤b,ZoharItzhaki¤c,HanahMargalit* DepartmentofMicrobiologyandMolecularGenetics,IMRIC,FacultyofMedicine,TheHebrewUniversityofJerusalem,Jerusalem,Israel Abstract Manyprotein-proteininteractionsaremediatedbydomain-motifinteraction,whereadomaininoneproteinbindsashort linearmotifinitsinteractingpartner.Suchinteractionsareofteninvolvedinkeycellularprocesses,necessitatingtheirtight regulation.Acommonstrategyofthecelltocontrolproteinfunctionandinteractionisbypost-translationalmodifications of specific residues, especially phosphorylation. Indeed, there are motifs, such as SH2-binding motifs, in which motif phosphorylation is required for the domain-motif interaction. On the contrary, there are other examples where motif phosphorylationpreventsthedomain-motifinteraction.Herewepresentalarge-scaleintegrativeanalysisofexperimental humandataofdomain-motifinteractionsandphosphorylationevents,demonstratinganintriguingcouplingbetweenthe two.WereportsuchcouplingforSH3,PDZ,SH2andWWdomains,whereresiduephosphorylationwithinornexttothe motifisimpliedtobeassociatedwithswitchingonoroffdomainbinding.Fordomainsthatrequiremotifphosphorylation for binding, such as SH2 domains, we found coupled phosphorylation events other than the ones required for domain binding.Furthermore,weshowthatphosphorylationmightfunctionasadoubleswitch,concurrentlyenablinginteraction ofthemotifwithonedomainanddisablinginteractionwithanotherdomain.Evolutionaryanalysisshowsthatco-evolution of the motif and the proximal residues capable of phosphorylation predominates over other evolutionary scenarios, in whichthemotifappearedbeforethepotentiallyphosphorylatedresidue,orviceversa.Ourfindingsprovidestrengthening evidenceforcoupledinteraction-regulationunits,definedbyadomain-bindingmotifandaphosphorylatedresidue. Citation: Akiva E, Friedlander G, Itzhaki Z, Margalit H (2012) A Dynamic View of Domain-Motif Interactions. PLoS Comput Biol 8(1): e1002341. doi: