Activation of Endogenous FAK via Expression of Its Amino Terminal Domain in Xenopus Embryos 英文参考文献.docVIP

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Activation of Endogenous FAK via Expression of Its Amino Terminal Domain in Xenopus Embryos 英文参考文献.doc

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Activation of Endogenous FAK via Expression of Its Amino Terminal Domain in Xenopus Embryos 英文参考文献

ActivationofEndogenousFAKviaExpressionofIts AminoTerminalDomaininXenopusEmbryos NicolettaI.Petridou1.,PanayiotaStylianou1.,NeophytosChristodoulou1,DanielRhoads1 ,Jun- LinGuan2,ParisA.Skourides1* 1DepartmentofBiologicalSciences,UniversityofCyprus,Nicosia,Cyprus,2DepartmentofInternalMedicine-MMG,UniversityofMichiganMedicalSchool,AnnArbor, Michigan,UnitedStatesofAmerica Abstract Background:TheFocalAdhesionKinaseisawellstudiedtyrosinekinaseinvolvedinawidenumberofcellularprocesses includingcelladhesionandmigration.Ithasalsobeenshowntoplayimportantrolesduringembryonicdevelopmentand targeteddisruptionoftheFAKgeneinmiceresultsinembryoniclethalitybyday8.5. PrincipalFindings:HereweexaminedthepatternofphosphorylationofFAKduringXenopusdevelopmentandfoundthat FAKisphosphorylatedonallmajortyrosineresiduesexaminedfromearlyblastulastageswellbeforeanymorphogenetic movementstakeplace.WegoontoshowthatFRNKfailstoactasadominantnegativeinthecontextoftheearlyembryo andthattheFERMdomainhasamajorroleindeterminingFAK’slocalizationattheplasmamembrane.Finally,weshowthat autonomous expression of the FERM domain leads to the activation of endogenous FAK in a tyrosine 397 dependent fashion. Conclusions:Overall,ourdatasuggestanimportantrolefortheFERMdomainintheactivationofFAKandindicatethat integrinsignallingplaysalimitedroleintheinvivoactivationofFAKatleastduringtheearlystagesofdevelopment. Citation:PetridouNI,StylianouP,ChristodoulouN,RhoadsD,GuanJ-L,etal.(2012)ActivationofEndogenousFAKviaExpressionofItsAminoTerminalDomain inXenopusEmbryos.PLoSONE7(8):e42577.doi:10.1371/journal.pone.0042577 Editor:EffieC.Tsilibary,NationalCenterforScientificResearchDemokritos,Greece ReceivedMarch29,2012;AcceptedJuly9,2012;PublishedAugust6,2012 Copyright: ? 2012 Petridou et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding: Funding