Adipose Tissue Gene Expression of Factors Related to Lipid Processing in Obesity 英文参考文献.docVIP

AdiposeTissueGeneExpressionofFactorsRelatedto LipidProcessinginObesity MercedesClemente-Postigo1,MariaIsabelQueipo-Ortun?o1,2,DiegoFernandez-Garcia2,3 ,Ricardo Gomez-Huelgas2,4,FranciscoJ.Tinahones2,3,FernandoCardona1,2 * 1LaboratoriodeInvestigacionesBiome′dicas delHospitalVirgendelaVictoria,Ma′laga (Fundacio′nIMABIS),Spain,2CIBERFisiopatolog?′adelaObesidadylaNutricio′n, Spain,3ServicioEndocrinolog?′ayNutricio′ndelHospitalUniversitarioVirgen delaVictoria,Ma′laga, Spain,4ServiciodeMedicinaInternadelHospitalRegional Carlos Haya,Spain Abstract Background: Adipose tissue lipid storage and processing capacity can be a key factor for obesity-related metabolic disorderssuchasinsulinresistanceanddiabetes.Lipiduptakeisthefirststeptoadiposetissuelipidstorage.Theaimofthis study was to analyze the gene expression of factors involved in lipid uptake and processing in subcutaneous (SAT) and visceral(VAT)adiposetissueaccordingtobodymassindex(BMI)andthedegreeofinsulinresistance(IR). MethodsandPrincipalFindings:VLDLreceptor(VLDLR),lipoproteinlipase(LPL),acylationstimulatingprotein(ASP),LDL receptor-relatedprotein1(LRP1)andfattyacidbindingprotein4(FABP4)geneexpressionwasmeasuredinVATandSAT from28morbidlyobesepatientswithType2DiabetesMellitus(T2DM)orhighIR,10morbidlyobesepatientswithlowIR, 10obesepatientswithlowIRand12leanhealthycontrols.LPL,FABP4,LRP1andASPexpressioninVATwashigherinlean controls. In SAT, LPL and FABP4 expression were also higher in lean controls. BMI, plasma insulin levels and HOMA-IR correlated negatively with LPL expression in both VAT and SAT as well as with FABP4 expression in VAT. FABP4 gene expressioninSATcorrelatedinverselywithBMIandHOMA-IR.However,multipleregressionanalysisshowedthatBMIwas themainvariablecontributingtoLPLandFABP4geneexpressioninbothVATandSAT. Conclusions:Morbidlyobesepatientshavealowergeneexpressionoffactorsrelatedwithlipiduptakeandprocessingin comparisonwithhealthyleanpersons. Citation:Clemente-PostigoM,Queipo-Ortun?oMI,Ferna