Adrenocorticotropic Hormone Suppresses Gonadotropin-Stimulated Estradiol Release from Zebrafish Ovarian Follicles 英文参考文献.docVIP

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Adrenocorticotropic Hormone Suppresses Gonadotropin-Stimulated Estradiol Release from Zebrafish Ovarian Follicles 英文参考文献.doc

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Adrenocorticotropic Hormone Suppresses Gonadotropin-Stimulated Estradiol Release from Zebrafish Ovarian Follicles 英文参考文献

AdrenocorticotropicHormoneSuppresses Gonadotropin-StimulatedEstradiolReleasefrom ZebrafishOvarianFollicles DerekAlsop¤,JenniferS.Ings,MathilakathM.Vijayan* DepartmentofBiology,UniversityofWaterloo,Waterloo,Ontario,Canada Abstract While stress is known to impact reproductive performance, the pathways involved are not entirely understood. Corticosteroid effects on the functioning of the hypothalamus-pituitary-gonadal axis are thought to be a key aspect of stress-mediated reproductive dysfunction. A vital component of the stress response is the pituitary secretion of adrenocorticotropic hormone (ACTH), which binds to the melanocortin 2 receptor (MC2R) in the adrenal glands and activatescortisolbiosynthesis.WerecentlyreportedMC2RmRNAabundanceinfishgonadsleadingtothehypothesisthat ACTHmaybedirectlyinvolvedingonadalsteroidmodulation.Usingzebrafish(Daniorerio)ovarianfollicles,wetestedthe hypothesisthatacuteACTHstimulationmodulatescortisolandestradiol(E2)secretion.ACTHneitheraffectedcortisolnor unstimulatedE2releasefromovarianfollicles.However,ACTHsuppressedhumanchorionicgonadotropin(hCG)-stimulated E2secretioninadose-relatedmanner,withamaximumdecreaseof62%observedat1I.U.ACTHmL21.ThiseffectofACTH on E2 release was not observed in the presence of either 8-bromo-cAMP or forskolin, suggesting that the mechanism(s) involved in steroid attenuation was upstream of adenylyl cyclase activation. Overall, our results suggest that a stress- inducedriseinplasmaACTHlevelsmayinitiatearapiddown-regulationofacutestimulatedE2biosynthesisinthezebrafish ovary,underscoringanovelphysiologicalroleforthispituitarypeptideinmodulatingreproductiveactivity. Citation: Alsop D, Ings JS, Vijayan MM (2009) Adrenocorticotropic Hormone Suppresses Gonadotropin-Stimulated Estradiol Release from Zebrafish Ovarian Follicles.PLoSONE4(7):e6463.doi:10.1371/journal.pone.0006463 Editor:RyanL.Earley,UniversityofAlabama,UnitedStatesofAmerica ReceivedMarch25,2009;AcceptedJune30,2009;PublishedJuly31,2009 Copyright: