Alteration of Proteins and Pigments Influence the Function of Photosystem I under Iron Deficiency from Chlamydomonas reinhardtii 英文参考文献.docVIP

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Alteration of Proteins and Pigments Influence the Function of Photosystem I under Iron Deficiency from Chlamydomonas reinhardtii 英文参考文献.doc

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Alteration of Proteins and Pigments Influence the Function of Photosystem I under Iron Deficiency from Chlamydomonas reinhardtii 英文参考文献

AlterationofProteinsandPigmentsInfluencethe FunctionofPhotosystemIunderIronDeficiencyfrom Chlamydomonasreinhardtii VenkateswarluYadavalli1,CraigC.Jolley2¤,ChandramouliMalleda1,BalakumarThangaraj2, PetraFromme2,RajagopalSubramanyam3* 1DepartmentofBiochemistry,SchoolofLifeSciences,UniversityofHyderabad,Hyderabad,India,2DepartmentofChemistryandBiochemistry,CenterforBioenergyand Photosynthesis,ArizonaStateUniversity, Tempe,Arizona,United States ofAmerica, 3Department ofPlant Sciences, School ofLifeSciences,University ofHyderabad, Hyderabad,India Abstract Background: Iron is an essential micronutrient for all organisms because it is a component of enzyme cofactors that catalyzeredoxreactionsinfundamentalmetabolicprocesses.Eventhoughironisabundantonearth,itisoftenpresentin theinsolubleferric[Fe(III)]state,leavingmanysurfaceenvironmentsFe-limited.ThehaploidgreenalgaChlamydomonas reinhardtiiisusedasamodelorganismforstudyingeukaryoticphotosynthesis.Thisstudyexploresstructuralandfunctional changes in PSI-LHCI supercomplexes under Fe deficiency as the eukaryotic photosynthetic apparatus adapts to Fe deficiency. Results:77KemissionspectraandsucrosedensitygradientdatashowthatPSIandLHCIsubunitsareaffectedunderiron deficiency conditions. The visible circular dichroism (CD) spectra associated with strongly-coupled chlorophyll dimers increases in intensity. The change in CD signals of pigments originates from the modification of interactions between pigmentmolecules.Evidencefromsucrosegradientsandnon-denaturing(green)gelsindicatesthatPSI-LHCIlevelswere reduced after cells were grown for 72h in Fe-deficient medium. Ultrafast fluorescence spectroscopy suggests that red- shifted pigments in the PSI-LHCI antenna were lost during Fe stress. Further, denaturing gel electrophoresis and immunoblotanalysisrevealsthatlevelsofthePSIsubunitsPsaCandPsaDdecreased,whilePsaEwascompletelyabsent afterFestress.Thelightharvestingcomplexeswerealsosusceptibletoirondeficiency,withLhca1andLhca9showing