Altered Neuronal Intrinsic Properties and Reduced Synaptic Transmission of the Rats Medial Geniculate Body in Salicylate-Induced Tinnitus 英文参考文献.docVIP

AlteredNeuronalIntrinsicPropertiesandReduced SynapticTransmissionoftheRat’sMedialGeniculate BodyinSalicylate-InducedTinnitus Yan-YanSu1,2.,BinLuo1,2.,YanJin1,2,Shu-HuiWu3,EdwardLobarinas4,RichardJ.Salvi4,LinChen1,2* 1CASKeyLaboratoryofBrainFunctionandDisease,SchoolofLifeSciences,UniversityofScienceandTechnologyofChina,Hefei,China,2AuditoryResearchLaboratory, University of Science and Technology of China, Hefei, China, 3Department of Neuroscience, Carleton University, Ottawa, Ontario, Canada, 4Center for Hearing and Deafness,StateUniversityofNewYorkatBuffalo,Buffalo,NewYork,UnitedStatesofAmerica Abstract Sodium salicylate (NaSal), an aspirin metabolite, can cause tinnitus in animals and human subjects. To explore neural mechanisms underlying salicylate-induced tinnitus, we examined effects of NaSal on neural activities of the medial geniculate body (MGB), an auditory thalamic nucleus that provides the primary and immediate inputs to the auditory cortex,byusingthewhole-cellpatch-clamprecordingtechniqueinMGBslices.RatstreatedwithNaSal(350mg/kg)showed tinnitus-like behavior as revealed by the gap prepulse inhibition of acoustic startle (GPIAS) paradigm. NaSal (1.4mM) decreased the membrane input resistance, hyperpolarized the resting membrane potential, suppressed current-evoked firing, changed the action potential, and depressed rebound depolarization in MGB neurons. NaSal also reduced the excitatoryandinhibitorypostsynapticresponseintheMGBevokedbystimulatingthebrachiumoftheinferiorcolliculus. OurresultsdemonstratethatNaSalaltersneuronalintrinsicpropertiesandreducesthesynaptictransmissionoftheMGB, whichmaycauseabnormalthalamicoutputstotheauditorycortexandcontributetoNaSal-inducedtinnitus. Citation:SuY-Y,LuoB,JinY,WuS-H,LobarinasE,etal.(2012)AlteredNeuronalIntrinsicPropertiesandReducedSynaptic TransmissionoftheRat’sMedial GeniculateBodyinSalicylate-InducedTinnitus.PLoSONE7(10):e46969.doi:10.1371/journal.pone.0046969 Editor:ManuelS.Malmierca,UniversityofSala