An ATP and Oxalate Generating Variant Tricarboxylic Acid Cycle Counters Aluminum Toxicity in Pseudomonas fluorescens 英文参考文献.docVIP

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An ATP and Oxalate Generating Variant Tricarboxylic Acid Cycle Counters Aluminum Toxicity in Pseudomonas fluorescens 英文参考文献.doc

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An ATP and Oxalate Generating Variant Tricarboxylic Acid Cycle Counters Aluminum Toxicity in Pseudomonas fluorescens 英文参考文献

AnATPandOxalateGeneratingVariantTricarboxylic AcidCycleCountersAluminumToxicityinPseudomonas fluorescens RanjiSingh,JosephLemire,RyanJ.Mailloux,DanielChe′nier,RobertHamel,VasuD.Appanna* DepartmentofChemistryandBiochemistry,LaurentianUniversity,Sudbury,Ontario,Canada Abstract Although the tricarboxylic acid (TCA) cycle is essential in almost all aerobic organisms, its precise modulation and integrationinglobalcellularmetabolismisnotfullyunderstood.Here,wereportonanalternativeTCAcycleuniquelyaimed at generating ATP and oxalate, two metabolites critical for the survival of Pseudomonas fluorescens. The upregulation of isocitrate lyase (ICL) and acylating glyoxylate dehydrogenase (AGODH) led to the enhanced synthesis of oxalate, a dicarboxylicacidinvolvedintheimmobilizationofaluminum(Al).Theincreasedactivityofsuccinyl-CoAsynthetase(SCS) andoxalateCoA-transferase(OCT)intheAl-stressedcellsaffordedaneffectiveroutetoATPsynthesisfromoxalyl-CoAvia substratelevelphosphorylation.ThismodifiedTCAcyclewithdiminishedefficacyinNADHproductionanddecreasedCO2- evolvingcapacity,orchestratesthesynthesisofoxalate,NADPH,andATP,ingredientspivotaltothesurvivalofP.fluorescens inanAlenvironment. Thechannelingofsuccinyl-CoAtowardsATPformationmaybeanimportantfunctionoftheTCA cycleduringanaerobiosis,FestarvationandO2-limitedconditions. Citation:SinghR,LemireJ,MaillouxRJ,Che′nierD,HamelR,etal.(2009)AnATPandOxalateGeneratingVariantTricarboxylicAcidCycleCountersAluminum ToxicityinPseudomonasfluorescens.PLoSONE4(10):e7344.doi:10.1371/journal.pone.0007344 Editor:MichaelC.Lorenz,UniversityofTexas-HoustonMedicalSchool,UnitedStatesofAmerica ReceivedJuly17,2009;AcceptedSeptember11,2009;PublishedOctober7,2009 Copyright: ? 2009 Singh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisworkwasfundedbytheNorthernOntarioHer