Amplification of a Cytochrome P450 Gene Is Associated with Resistance to Neonicotinoid Insecticides in the Aphid Myzus persicae 英文参考文献.docVIP
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Amplification of a Cytochrome P450 Gene Is Associated with Resistance to Neonicotinoid Insecticides in the Aphid Myzus persicae 英文参考文献
AmplificationofaCytochromeP450GeneIsAssociated
withResistancetoNeonicotinoidInsecticidesinthe
AphidMyzuspersicae
AlinM.Puinean1,2,StephenP.Foster1,LindaOliphant1,IanDenholm1,LindaM.Field1,NeilS.Millar2,
MartinS.Williamson1,ChrisBass1*
1CentreforSustainablePestandDiseaseManagement,RothamstedResearch,Harpenden,UnitedKingdom,2ResearchDepartmentofNeuroscience,Physiology,and
Pharmacology,UniversityCollegeLondon,London,UnitedKingdom
Abstract
TheaphidMyzuspersicaeisagloballysignificantcroppestthathasevolvedhighlevelsofresistancetoalmostallclassesof
insecticide. To date, the neonicotinoids, an economically important class of insecticides that target nicotinic acetylcholine
receptors(nAChRs),haveremainedaneffectivecontrolmeasure;however,recentreportsofresistanceinM.persicaerepresenta
threattothelong-termefficacyofthischemicalclass.Inthisstudy,themechanismsunderlyingresistancetotheneonicotinoid
insecticideswereinvestigatedusingbiological,biochemical,andgenomicapproaches.BioassaysonaresistantM.persicaeclone
(5191A)suggestedthatP450-mediateddetoxificationplaysaprimaryroleinresistance,althoughadditionalmechanism(s)may
alsocontribute.Microarrayanalysis,usinganarraypopulatedwithprobescorrespondingtoallknowndetoxificationgenesinM.
persicae,revealedconstitutiveover-expression(22-fold)ofasingleP450gene(CYP6CY3);andquantitativePCRshowedthatthe
over-expressionisdue,atleastinpart,togeneamplification.ThisisthefirstreportofaP450geneamplificationeventassociated
withinsecticideresistanceinanagriculturallyimportantinsectpest.Themicroarrayanalysisalsoshowedover-expressionof
severalgenesequencesthatencodecuticularproteins(2–16-fold),andartificialfeedingassaysandinvivopenetrationassays
usingradiolabeledinsecticideprovideddirectevidenceofaroleforreducedcuticularpenetrationinneonicotinoidresistance.
Conversely,receptorradioligandbindingstudiesandnucleotidesequencingofnAChRsubunitgenessuggestthattarget-site
changesareunlikelytocontributetoresistancetoneonicotinoidinsecticidesinM.persicae.
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